3h cck 8

Manufactured by PerkinElmer

[3H]-CCK-8 is a radiolabeled ligand used in research applications. It is a synthetic derivative of the peptide cholecystokinin, with a tritium label. This product can be used in binding studies and other experimental procedures involving the cholecystokinin receptor.

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CCK-8 (23 citations) [3H]-cholecystokinin-8 (CCK-8) (4 citations)

2 protocols using 3h cck 8

Radiolabeled CCK-8 and E2G Transport Assay

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[³H]-CCK-8 and [³H]-E₂G were obtained from PerkinElmer (Boston, MA), while non-radiolabeled CCK-8 and E₂G were purchased from the Peptide Institute (Osaka, Japan) and Sigma, respectively. The transport assay was performed using LS180 and HCT116 cells based essentially on the previously described method [17 (link)]. Final concentrations of CCK-8 and E₂G were 1 μM and 0.5 μM, respectively. The transport activity for each substrate was determined within a linear range (5 min). In the inhibition analysis, bromosulfophthalein (BSP, Sigma) (100 μM) was used.

Sun Y., Furihata T., Ishii S., Nagai M., Harada M., Shimozato O., Kamijo T., Motohashi S., Yoshino I., Kamiichi A., Kobayashi K, & Chiba K. (2014). Unique expression features of cancer-type organic anion transporting polypeptide 1B3 mRNA expression in human colon and lung cancers. Clinical and Translational Medicine, 3, 37.

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OATP1B Inhibitory Potential of Drugs

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RST, E 2 17bG, and CCK8 (Sigma) were used as substrates in this study. For radiolabeled tracing, [ 3 H]-E 2 17bG and [ 3 H]-CCK8 were used, which were both purchased from PerkinElmer Life and Analytical Sciences. OATP1B inhibitory potential of 22 drugs was investigated. Sixteen of these 22 drugs (i.e., baicalin, cyclosporine A, darunavir, digoxin, erythromycin, ezetimibe, fluconazole, gemfibrozil, grazoprevir, ketoconazole, lopinavir, metformin, rifampicin, ritonavir, telmisartan, and valsartan) were purchased from Sigma; three (i.e., atazanavir, fimasartan, and velpatasvir) were purchased from Selleckchem; and asunaprevir, eltrombopag, and eluxadoline were purchased from Carbosynth, SeqChem, and Toronto Research Chemicals, respectively.

Sane R., Cheung K.W.K., Kovács P., Farasyn T., Li R., Bui A., Musib L., Kis E., Plise E, & Gáborik Z. (2020). Calibrating the In Vitro-In Vivo Correlation for OATP-Mediated Drug-Drug Interactions with Rosuvastatin Using Static and PBPK Models. Drug metabolism and disposition: the biological fate of chemicals, 48(12).

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