Total RNA was extracted from the spleen samples with the RNeasy Mini Spin Kit (TAKARA, Dalian, China), and cDNA was prepared with the SuperScript RT Reagent Kit (TAKARA). SYBR chemistry (TAKARA) was used to amplify PCR; primers were designed with Primer Express software (Applied Biosystems, Foster City, CA, USA) and listed in Table 1 . The PCR was performed with the Roche Lightcycler 2.0 (Basel, Switzerland).
Sybr chemistry
Manufactured by Takara Bio
Sourced in United States, Japan
SYBR chemistry is a fluorescent dye-based detection method used in real-time PCR (polymerase chain reaction) to quantify DNA and RNA. It binds to double-stranded DNA, and the fluorescence emitted is detected and measured by the real-time PCR instrument to determine the amount of target nucleic acid present in the sample.
Automatically generated - may contain errors
2 protocols using sybr chemistry
1
Spleen RNA Extraction and qPCR Analysis
2
Angiotensinogen Gene Expression Analysis
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