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Glutamax 1 gibco

Manufactured by Thermo Fisher Scientific

GlutaMAX-I Gibco is a cell culture supplement that provides a stable source of L-glutamine. It is designed to support cell growth and function in various cell culture applications.

Automatically generated - may contain errors

3 protocols using glutamax 1 gibco

1

Transient Expression of Human Antibodies

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Example 3

Transient gene expression of human antibodies is achieved upon transfection of antibody expression vectors into 293-T human embryonic kidney cells or Chinese Hamster Ovary cells (CHO) using the Polyethylenimine Transfection method (PEI, Polyscience Warrington, USA). After transfection cells are cultured in serum free medium (OPTI-MEM I supplemented with GlutaMAX-I Gibco). Supernatants are collected after 3-6 days of culture and IgG is purified using protein A columns (GE HealthCare, Sweden) on a fast protein liquid chromatography device (FPLC) (GE HealthCare, Sweden).

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2

Transient Expression of Human Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 5

Transient gene expression of human antibodies is achieved upon transfection of antibody expression vectors into 293-T human embryonic kidney cells or Chinese Hamster Ovary cells (CHO) using the Polyethylenimine Transfection method (PEI, Polyscience Warrington, USA). After transfection cells are cultured in serum free medium (OPTI-MEM I supplemented with GlutaMAX-I Gibco). Supernatants are collected after 3-6 days of culture and IgG is purified using protein A columns (GE HealthCare, Sweden) on a fast protein liquid chromatography device (FPLC) (GE HealthCare, Sweden).

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3

Transient Antibody Expression in 293-T and CHO Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 3

Transient gene expression of human antibodies is achieved upon transfection of antibody expression vectors into 293-T human embryonic kidney cells or Chinese Hamster Ovary cells (CHO) using the Polyethylenimine Transfection method (PEI, Polyscience Warrington, USA). After transfection cells are cultured in serum free medium (OPTI-MEM I supplemented with GlutaMAX-I Gibco). Supernatants are collected after 3-6 days of culture and IgG is purified using protein A columns (GE HealthCare. Sweden) on a fast protein liquid chromatography device (FPLC) (GE HealthCare, Sweden).

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