Basic fibroblast growth factor fgf2

Manufactured by Thermo Fisher Scientific

Sourced in United States

Basic fibroblast growth factor (FGF2) is a protein that stimulates the growth and differentiation of various cell types, including fibroblasts, endothelial cells, and neural cells. It is involved in the regulation of cell proliferation, migration, and survival.

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Lab products found in correlation

2 mercaptoethanol β mee, by Merck Group (1 mentions) Dmem f12, by Fujifilm (1 mentions) L glutamine, by Fujifilm (1 mentions) Knockout serum replacement (ksr), by Thermo Fisher Scientific (1 mentions) Laminin, by Thermo Fisher Scientific (1 mentions) Rencell nsc maintenance medium, by Merck Group (1 mentions) Cell lysis buffer for western and ip, by Beyotime (1 mentions) Lactate dehydrogenase assay kit, by Beyotime (1 mentions) Malondialdehyde assay kit, by Beyotime (1 mentions) Total superoxide dismutase assay kit, by Dojindo Laboratories (1 mentions) Real time pcr kit, by Tiangen Biotech (1 mentions) Goat anti mouse igg hrp, by Beyotime (1 mentions) Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions) Goat anti rabbit igg hrp, by Beyotime (1 mentions) Catalase assay kit, by Beyotime (1 mentions) Accutase, by Merck Group (1 mentions) Bca protein assay kit, by Beyotime (1 mentions) Tripure, by Roche (1 mentions) N acetylcysteine nac, by Merck Group (1 mentions) C57bl 6 tg cag egfp 10sb j mice, by Jackson ImmunoResearch (1 mentions)

3 protocols using basic fibroblast growth factor fgf2

Human iPS Cell Culture Protocol

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Human iPS cell line Windy was provided by Dr. Akihiro Umezawa, National Center for Child Health and Development. Cells were cultured on mitomycin C-treated mouse embryonic fibroblasts in a 1:1 mixture of Dulbecco’s modified Eagle’s medium (DMEM) and Ham’s nutrient mixture F-12 (DMEM/F-12) (Wako Pure Chemical Industries, Osaka, Japan) basal medium with 20% KnockOut Serum Replacement (Thermo Fisher Scientific, Carlsbad, CA, USA), 2 mM l-glutamine (Wako Pure Chemical Industries), 1% minimum essential medium nonessential amino acid solution (NEAA) (Wako Pure Chemical Industries), 0.1 mM 2-mercaptoethanol (β-MeE) (Sigma-Aldrich, St. Louis, MO, USA), and 5 ng/mL basic fibroblast growth factor (FGF2) (PeproTech Inc., Rocky Hill, NJ, USA).

Qiu S., Kabeya T., Ogawa I., Anno S., Hayashi H., Kanaki T., Hashita T., Iwao T, & Matsunaga T. (2020). Gellan Gum Promotes the Differentiation of Enterocytes from Human Induced Pluripotent Stem Cells. Pharmaceutics, 12(10), 951.

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Neural stem cell culture protocol

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PQ was purchased from Sigma Chemical Co. (Sigma-Aldrich, Milan, Italy). ReNcell NSC Maintenance Medium and accutase were obtained commercially from Millipore (Temecula, CA). Epidermal growth factor (EGF) and basic fibroblast growth factor (FGF-2) were purchased from PeproTech. Laminin was purchased from Invitrogen (Carlsbad, CA, USA). Catalase Assay Kit, Malondialdehyde Assay Kit, Lactate Dehydrogenase Assay Kit, BCA Protein Assay Kit, Cell Lysis Buffer for Western and IP, goat anti-rabbit IgG-HRP, and goat anti-mouse IgG-HRP were obtained from Beyotime (Jiangsu, China). Total Superoxide Dismutase Assay Kit was purchased from Dojindo Molecular Technologies (Kumamoto, Japan). Tripure was obtained from Roche (Basel, Switzerland). The AMV first strand cDNA Synthesis Kit was purchased from MBI (Fermentas, Canada). Real-time PCR Kit was obtained from Tiangen Biotech (Beijing, China). Rabbit anti-Nrf2 polyclonal antibody, rabbit anti-Keap1 polyclonal antibody, rabbit anti-PKC polyclonal antibody, and rabbit anti-CKII polyclonal antibody were purchased from GeneTex (San Antonio, USA). Mouse anti-β-tubulin polyclonal antibody was purchased from Boster (Wuhan, China).

Dou T., Yan M., Wang X., Lu W., Zhao L., Lou D., Wu C., Chang X, & Zhou Z. (2015). Nrf2/ARE Pathway Involved in Oxidative Stress Induced by Paraquat in Human Neural Progenitor Cells. Oxidative Medicine and Cellular Longevity, 2016, 8923860.

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Isolation and Expansion of Embryonic Spinal Progenitors

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All animal work pertaining to progenitor cell isolation was performed with prior approval and in accordance with the Institutional Animal Care and Use Committee (IACUC) guidelines at the University of Michigan and University of Miami. Embryonic spinal progenitors were isolated from the spinal cords of embryonic day 14 (E14) C57BL/6-Tg(CAG-EGFP)10sb/J mice (Jackson Laboratory, Bar Harbor, ME), enzymatically dissociated with 10 U/mL papain (Worthington, Lakewood, NY) and 37 μg/mL DNase (Sigma) into single cells, and expanded as neurospheres in ultralow attachment flasks (Corning, Corning, NY), as described previously (Dumont et al., 2018 (link)). Embryonic spinal progenitors were expanded in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, Grand Island, NY) supplemented with 1X B27 (Gibco), 1X N2 (Gibco), N-acetyl cysteine (NAC; Sigma), and 20 ng/mL basic fibroblast growth factor (FGF2; Peprotech, Rocky Hill, NJ) and leukemia inhibitory factor (LIF; Peprotech). E14 cell colonies were passaged with papain as needed and not used beyond the second passage for transplantation studies.

Ciciriello A.J., Smith D.R., Munsell M.K., Boyd S.J., Shea L.D, & Dumont C.M. (2021). IL-10 lentivirus-laden hydrogel tubes increase spinal progenitor survival and neuronal differentiation after spinal cord injury. Biotechnology and bioengineering, 118(7), 2609-2625.

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