Ctgf antibody

Manufactured by Abcam

Sourced in United States

The CTGF antibody is a laboratory reagent used to detect the CTGF (Connective Tissue Growth Factor) protein in biological samples. CTGF is a secreted protein involved in various cellular processes, including cell proliferation, differentiation, and extracellular matrix production. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and ELISA to study the expression and localization of the CTGF protein.

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Lab products found in correlation

Acetylated α tubulin, by Santa Cruz Biotechnology (1 mentions) Histone h3, by Merck Group (1 mentions) α tubulin, by Merck Group (1 mentions) Acetylated histone h3, by Cell Signaling Technology (1 mentions) Hdac2 antibody, by Santa Cruz Biotechnology (1 mentions) Gapdh, by Biosynth (1 mentions) Gapdh antibody, by Cell Signaling Technology (1 mentions) Yap antibody, by Cell Signaling Technology (1 mentions) Piezo1 antibody, by Affinity Biosciences (1 mentions) Hrp conjugated goat anti rabbit antibody, by Cell Signaling Technology (1 mentions) α sma antibody, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Anti-CTGF antibody (5 citations) Mouse monoclonal anti-CTGF antibody (3 citations)

2 protocols using ctgf antibody

Western Blot Analysis of LV Myocardial Samples

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LV myocardial samples were homogenized in lysis buffer and supernatant and pellet fractions for each sample run on SDS-PAGE and Western blotting was performed using CTGF antibody (1:2500, Abcam, cat# ab6992), acetylated histone H3 (Cell Signaling #9649), histone H3 (Millipore 06-755), acetylated α-Tubulin (Santa Cruz 23950) and α-Tubulin (Sigma, T9026). Band intensity was quantified using ImageJ 1.47t software (NIH, USA, http://imagej.nih.gov/ij/).
Protein samples (5µg) from DCM cells (siRNA experiment) were run on 4–12% bis-tris gel and Western blotting was performed using HDAC2 antibody (Santa Cruz, cat# sc7899) and GAPDH (Fitzgerald Industries International, cat# 10R-G109a).

Renaud L., Harris L.G., Mani S.K., Kasiganesan H., Chou J.C., Baicu C.F., Van Laer A., Akerman A.W., Stroud R.E., Jones J.A., Zile M.R, & Menick D.R. (2015). HDACs Regulate miR-133a Expression in Pressure Overload Induced Cardiac Fibrosis. Circulation. Heart failure, 8(6), 1094-1104.

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Western Blot Analysis of Piezo1 and Signaling

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The acquired tissues and cells were lysed with RIPA on ice for 30 min. Then, proteins were harvested by a low temperature high speed centrifuge for 10 min. The primary antibodies used in our study include Piezo1 antibody (1:1000; Affinity, DF12083, China), GAPDH antibody (1:1000; Cell Signaling Technology, 2118, USA), α-SMA antibody (1:1000; Cell Signaling Technology, 19245, USA), pMAPK antibody (1:1000; Cell Signaling Technology, 9101, USA), CTGF antibody (1:1000; Abcam, ab6992, England), and YAP antibody (1:1000; Cell Signaling Technology, 14074, USA). Goat anti-rabbit HRP-conjugated antibody (1:5000; Cell Signaling Technology, 7074, USA) was used as secondary antibody. Then the ultra-high sensitivity ECL kit (MedChemExpress, HY-K1005, USA) was used for chromogenic development for 2 min. The bands were detected by a chemiluminescence system (ChemiScope 3300 Mini, China). ImageJ program was used to quantify the exposed protein bands.

Niu L., Cheng B., Huang G., Nan K., Han S., Ren H., Liu N., Li Y., Genin G.M, & Xu F. (2022). A positive mechanobiological feedback loop controls bistable switching of cardiac fibroblast phenotype. Cell Discovery, 8, 84.

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