Paracetamol

The following reference chemicals were used in the experiments: butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), resazurin, 2,3,5-triphenyltetrazolium chloride (TTC), chloramphenicol, nystatin, quercetin, pyrogallol Cremophor EL, all provided by Sigma-Aldrich (St. Louis, MO, USA); ascorbic acid (AAc), purchased from Dinâmica (Diadema, São Paulo, Brazil); linoleic acid, obtained from Vetec (Duque de Caxias, Rio de Janeiro, Brazil); 2,2-diphenyl-1-picrylhydrazyl (DPPH), provided by FLUKA (St. Louis, MO, USA); silver sulphadiazine 2% (União Química, Brasília, Distrito Federal, Brasil); paracetamol (Johnson & Johnson, São José dos Campos, São Paulo, Brazil); dipyrone (Sanofi Aventis, São Paulo, Brazil); ketamin (Cristália, Itapira, São Paulo, Brazil); and xylazine (Intervet Schering-Plough, São Paulo, Brazil). Stock solutions of these chemicals were prepared with suitable solvent and dilutions were made fresh on the day of experiment. Mueller-Hinton Agar and Mueller-Hinton Broth culture mediums were both purchased of HiMedia (Mumbai, India) and prepared with sterile distilled water.

Animals were submitted to general anesthesia by intraperitoneal injection with
Ketamine Hydrochloride solution (Cetamin 10%, Syntec, 50 ml) and Xylazine
Hydrochloride (Xilazin 2%, Syntec, 10 ml) in the proportion of 0.7 ml/g of
Ketamine and 0.5 ml/g of Xylazine. First upper molars were extracted
bilaterally, and the alveoli were separated randomly in 4 groups (n=5) to
receive: blood clot; autogenous bone, BDX, and DHDM. It was gently accommodated
with an instrument compatible with the size of the socket. Autogenous bone was
harvested from calvaria of the same animals using a trephine bur. All animals
were submitted to the same procedures. BDX commercial product, purchased from an
authorized dealer (Bio-Oss® L: 1mm-2mm [0,5 g~1,5 cc; 2 g~ 6 cc], Geistlich
Pharma do Brasil).
Analgesic medication (Paracetamol - Johnson & Johnson® do Brasil) at a dose
of 15mg/kg in a single postoperative dose and were administrated. Euthanasia was
performed after 7, 14 and 28 days using an overdose with an association of
ketamine hydrochloride and xylazine hydrochloride. Maxilla were dissected and
initially stored formaldehyde solution for fixation. After seven days, the
pieces were removed from the formaldehyde solution and immersed in
98^o alcohol where they were kept until µCT analyses and
histological processing.