Huh7 cells were transfected with miR-199a-3p mimic, small interfering RNA for YAP1 (si-YAP1), pcDNA3.1 vectors containing the cDNA of YAP1 (pcDNA-YAP1), pcDNA-Jagged1, si-Jagged1 and their respective controls (Ribobio, Guangzhou, China) by using Lipofactamine 2000 (Invitrogen) according to the manufacturer’s instructions. To investigate whether introduction of miR-199a-3p could increase HCC cell sensitivity to the drug γ-secretase inhibitor n-[n-(3,5-difluorophenacetyl-l-alanyl)]-s-phenylglycinet-butyl ester (DAPT; Calbiochem, San Diego, CA, USA), Huh7 cells transfected with miR-control or miR-199a-3p mimic were treated with DAPT at a final concentration of 10 μM for 48 h.
Si jagged1
Manufactured by RiboBio
Sourced in China
Si-Jagged1 is a laboratory equipment product. It functions as a tool for researchers to study cellular signaling pathways related to the Jagged1 protein.
Automatically generated - may contain errors
2 protocols using si jagged1
1
Modulating Huh7 Cell Sensitivity to DAPT
2
Jagged 1 Silencing in MDA-T68 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The Jagged 1 siRNA constructs (si-NC, and si-Jagged 1)
were provided by RiboBio, China. The sequence of si-NC
was 5′-CCAUCUCCCGGUACAAAAUCUGCU-3′ and
si-Jagged 1 sequence was 5′-GCAAAGAAGCCGUGUGUAA-3′. After MDA-T68 cells were cultured to 50-60%
confluency in 6-well plates, they were subjection to transfection with siRNA using the Lipofectamine 2000 reagent
(Invitrogen, USA) according to the manufacturer’s guidelines.
were provided by RiboBio, China. The sequence of si-NC
was 5′-CCAUCUCCCGGUACAAAAUCUGCU-3′ and
si-Jagged 1 sequence was 5′-GCAAAGAAGCCGUGUGUAA-3′. After MDA-T68 cells were cultured to 50-60%
confluency in 6-well plates, they were subjection to transfection with siRNA using the Lipofectamine 2000 reagent
(Invitrogen, USA) according to the manufacturer’s guidelines.
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