8-week-old mice were anaesthetized with an intraperitoneal injection of a mixture of tiletamine and zolazepam (1:1, 2.25 mg per kg body weight) and xylazine hydrochloride (0.7 mg per kg body weight). AAV9-CjCas9 (2 × 1010 viral genome in 2 μl) was intravitreally injected using a Nanofil syringe with a 33 G blunt needle (World Precision Instruments Inc.) under an operating microscope (Leica Microsystems Ltd.).
33 g blunt needle
Manufactured by World Precision Instruments
Sourced in United States
The 33 G blunt needle is a laboratory instrument used for precise fluid handling and sample transfer. It features a small 33-gauge diameter and a blunt tip to minimize sample disruption. The needle is designed for use with a variety of laboratory equipment and applications that require accurate and controlled fluid delivery.
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Lab products found in correlation
6 protocols using 33 g blunt needle
1
Intravitreal AAV9-CjCas9 Delivery in Mice
2
Subretinal CRISPR-Cas9 RNP Delivery
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Subretinal injection was performed as previously described (Park et al. 2015c (link)). Briefly, RNPs composed of Cas9 protein (8 µg), sgRNA (4.5 µg), and Lipofectamine 2000 (20% v/v) were mixed in 2 µL of injection volume. RNPs (2 or 3 µL) were injected into the subretinal space using a Nanofil syringe with a 33G blunt needle (World Precision Instruments, Inc.) under an operating microscope (Leica Microsystems, Ltd.). Subjects with retinal hemorrhage were excluded from the study.
3
Subretinal AAV Vector Delivery
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As previously described, after administration of deep anesthesia, injection of AAV-NT-ABEmax and AAV-CT-ABEmax (5.4 × 1010 viral genomes for AAV2/2 and 7.3 × 1010 viral genomes for AAV2/9 each in 3 μL of PBS) into the subretinal space of mice was performed using a customized Nanofil syringe with a 33G blunt needle (World Precision Instrument) under an operating microscope (Leica).48 (link)
4
Intravitreal Tie2 Kinase Inhibitor Dosage
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A specific Tie2 kinase inhibitor (TKI), CAS948557-43-5 (Merck & Millipore, Darmstadt, Germany), was dissolved with dimethylsulphoxide (DMSO) in the following concentrations: 0.01 mmol/L, 0.1 mmol/L, 1.0 mmol/L and 10.0 mmol/L. Then, 1 μl dilution was intravitreal injected with a 33 G blunt needle (World Precision Instruments Inc., Florida, USA) 3 days before the laser burns were placed around the optic disc. After comparing the effects and complications in the different dose groups, the 1.0 mmol/L concentration was selected in this study, and DMSO was set as the control.
5
Intravitreal Delivery of Genome-Editing Vectors
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Eight-week-old mice were anesthetized with an intraperitoneal injection of a mixture of tiletamine and zolazepam (1:1, 2.25 mg/kg body weight) and xylazine hydrochloride (0.7 mg/kg body weight). AAV2/9-LbCpf1-Vegfa or -Hif1a (2 × 1010 viral genomes in 2 μl) was intravitreally injected using a Nanofil syringe with a 33 G blunt needle (World Precision Instruments Inc.) under an operating microscope (Leica Microsystems Ltd.). The virus dose used in this study was limited due to production issues, and higher virus doses may elicit higher mutation efficacy.
6
AAV9-CCN5 Intravitreal Injection
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AAV9-CCN5 was intravitreally injected using a Nanofil syringe with a 33G blunt needle (World Precision Instruments Inc., USA) underneath an operating microscope (Leica Microsystems Ltd., Germany). The same concentration of AAV9-VLP was injected into control mice.
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