Log In Sign up
The largest database of trusted experimental protocols

Te2000 ti cube

Manufactured by Nikon
Visit Supplier

The Nikon TE2000/Ti cube is a modular inverted microscope system designed for advanced imaging applications. It features a compact, cube-shaped design and accommodates a range of microscope objectives, cameras, and accessories to support diverse research and analysis needs. The core function of the TE2000/Ti cube is to provide a stable and versatile platform for high-quality imaging and observation of samples.

Automatically generated - may contain errors

Lab products found in correlation

Poly d lysine coated glass bottom plates, by MatTek (2 mentions) Eclipse te2000 microscope, by Nikon (2 mentions) Plan apo objective, by Nikon (2 mentions)

Spelling variants of this product

TE2000 (456 citations) The Cube (2 citations)

2 protocols using te2000 ti cube

1

Live Imaging of Cell Reporters

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were plated in poly-D-lysine-coated glass-bottom plates (MatTek Corporation) and switched to phenol-red free culture medium supplemented with 10% FBS prior to live imaging. Cells were imaged using a Nikon Eclipse TE2000 microscope equipped with a chamber for controlled temperature (37%) and CO2 (5%) environment. All live-cell imaging was performed with a 10x Plan Apo objective (Nikon) and a Hamamatsu Orca ER camera using CFP, YFP, mCherry filter sets (Chroma). For EKAREV and EKAREN5 reporter imaging, the FRET signal was collected using customized ECFP/EYFP FRET filter sets with ET436/20x, ET535/30m, and T455lp mounting into the Nikon TE2000/Ti cube.
Eghbali B., Kessler J.A, & Spray D.C. (1990). Expression of gap junction channels in communication-incompetent cells after stable transfection with cDNA encoding connexin 32.. Proceedings of the National Academy of Sciences of the United States of America, 87(4), 1328-1331.
+ Open protocol
+ Expand
2

Live Imaging of Cell Reporters

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were plated in poly-D-lysine-coated glass-bottom plates (MatTek Corporation) and switched to phenol-red free culture medium supplemented with 10% FBS prior to live imaging. Cells were imaged using a Nikon Eclipse TE2000 microscope equipped with a chamber for controlled temperature (37%) and CO2 (5%) environment. All live-cell imaging was performed with a 10x Plan Apo objective (Nikon) and a Hamamatsu Orca ER camera using CFP, YFP, mCherry filter sets (Chroma). For EKAREV and EKAREN5 reporter imaging, the FRET signal was collected using customized ECFP/EYFP FRET filter sets with ET436/20x, ET535/30m, and T455lp mounting into the Nikon TE2000/Ti cube.
Chen J.Y., Hug C., Reyes J., Tian C., Gerosa L., Fröhlich F., Ponsioen B., Snippert H.J., Spencer S.L., Jambhekar A., Sorger P.K, & Lahav G. (2023). Multi-range ERK responses shape the proliferative trajectory of single cells following oncogene induction. Cell reports, 42(3), 112252.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!