Ab7208

Ten micrograms of proteins of each group were separated by 11 % SDS-PAGE and transferred onto nitrocellulose membranes. The blots were incubated with primary antibodies against human Nrf2(ab137550, Abcam. 1:400), TRAP-1 (ab2721, Abcam. 1:700), α-SMA (ab108424, Abcam. 1:500), FAP (ab227703, Abcam. 1:600), Collagen I (ab34710, Abcam.1:400), HDAC1 (ab7208, Abcam. 1:500), p-Nrf2 (ab76026, Abcam. 1:300) and β-actin (ab6276, Abcam. 1:2000) overnight at 4 °C, following incubated with the corresponding HRP-conjugated secondary antibody (ab205718, Abcam.1:3500). After washing the membranes twice with Tris Buffered Saline with Tween (TBST, pH = 7.4) and the bands in the membranes were visualized by enhanced chemiluminescence (ECL, Pierce, USA). After incubation for 2 min, the membranes were exposed to x-ray films. The protein bands were scanned and analyzed. The levels of these proteins were calculated as the ratio of the intensity of the specified protein to that of β-actin by using ImageJ2x software (National Institutes of Health, Maryland, USA). The intranuclear Nrf2 protein levels were calculated as the optical density ratio of intranuclear Nrf2 to HDAC1, and the phosphorylated Nrf2 was calculated as optical density ratio of phosphorylated Nrf2 to its total.

Ten micrograms of proteins of each group were separated by 11 % SDS-PAGE and transferred onto nitrocellulose membranes. The blots were incubated with primary antibodies against human Nrf2(ab137550, Abcam. 1:400), TRAP-1 (ab2721, Abcam. 1:700), α-SMA (ab108424, Abcam. 1:500), FAP (ab227703, Abcam. 1:600), Collagen I (ab34710, Abcam.1:400), HDAC1 (ab7208, Abcam. 1:500), p-Nrf2 (ab76026, Abcam. 1:300) and β-actin (ab6276, Abcam. 1:2000) overnight at 4 °C, following incubated with the corresponding HRP-conjugated secondary antibody (ab205718, Abcam.1:3500). After washing the membranes twice with Tris Buffered Saline with Tween (TBST, pH = 7.4) and the bands in the membranes were visualized by enhanced chemiluminescence (ECL, Pierce, USA). After incubation for 2 min, the membranes were exposed to x-ray films. The protein bands were scanned and analyzed. The levels of these proteins were calculated as the ratio of the intensity of the specified protein to that of β-actin by using ImageJ2x software (National Institutes of Health, Maryland, USA). The intranuclear Nrf2 protein levels were calculated as the optical density ratio of intranuclear Nrf2 to HDAC1, and the phosphorylated Nrf2 was calculated as optical density ratio of phosphorylated Nrf2 to its total.