LPS, ATP, nigericin, poly dA:dT, IAA94, and Duolink In Situ PLA kits were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ac-YVAD-cmk was obtained from Bachem (Torrance, CA, USA). The dye, 2′,7′-bis(2-carboxyethyl)-5,6-carboxyfluorescein acetoxymethyl ester (BCECF-AM), MitoSOX, MQAE (N-(Ethoxycarbonylmethyl)-6-Methoxyquinolinium Bromide) and DAPI were obtained from Invitrogen (Waltham, MA, USA). Flagellin (FLA-ST Ultrapure) was obtained from InvivoGen (San Diego, CA, USA). Alexa fluor 488TM Phalloidin was purchased from ThermoFisher Scientific. Abs were purchased for the detection of NLRP3 (Adipogen, San Diego, CA, USA), ASC (Adipogen), IL-1β (R&D systems, Minneapolis, MN, USA), caspase-1 (Adipogen), β-actin (Santa-Cruz Biotechnology, Dallas, TX, USA), and CLIC1 (Proteintech, Rosemont, IL, USA) Na+-K+ ATPase (Cell Signaling Technology, Danvers, MA, USA), and α-tubulin (Santa-Cruz Biotechnology).
Alexa fluor 488tm phalloidin
Manufactured by Thermo Fisher Scientific
Alexa Fluor® 488 Phalloidin is a high-affinity probe for F-actin. It is a conjugate of the mushroom toxin phalloidin and the Alexa Fluor® 488 fluorescent dye. Phalloidin binds tightly and specifically to F-actin, making it a useful tool for labeling and visualizing the actin cytoskeleton in cells.
Automatically generated - may contain errors
Lab products found in correlation
Ac yvad cmk, by Bachem (1 mentions)
Iaa94, by Merck Group (1 mentions)
Poly da dt, by Merck Group (1 mentions)
Duolink in situ pla kit, by Merck Group (1 mentions)
α tubulin, by Santa Cruz Biotechnology (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Adenosine triphosphate (atp), by Merck Group (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Nigericin, by Merck Group (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Flagellin fla st ultrapure, by InvivoGen (1 mentions)
Il 1β, by R&D Systems (1 mentions)
Caspase 1, by Adipogen (1 mentions)
Lsm 710 confocal microscope, by Zeiss (1 mentions)
Prolong antifade mountant, by Thermo Fisher Scientific (1 mentions)
Zen 2010, by Zeiss (1 mentions)
2 protocols using alexa fluor 488tm phalloidin
1
Inflammasome Activation Assay Protocol
2
Fluorescent Labeling of Neuronal F-Actin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cultured DRG neurons were fixed with 3% paraformaldehyde and 0.1% glutaraldehyde in PBS for 20 min and permeabilized with 0.1% Triton X-100 in PBS for 10 min. DRG neurons were then incubated with a high-affinity F-actin probe, Alexa Fluor 488TM phalloidin (1:40, ThermoFisher Scientific # A12379), in PBS for 20 min at RT, followed by a 5 min incubation with the nuclear counterstain DAPI (ThermoFisher Scientific # 62248). Samples were preserved using ProLong Antifade Mountant (ThermoFisher Scientific # P36961). Images were acquired on a Zeiss LSM710 confocal microscope with a 63x/1.4 Plan-Apochromat oil immersion objective. Images were processed using ZEN2010 software (Zeiss) and analyzed using Fiji ImageJ103 (link) (v.2.3.0/1.53q) to enhance contrast and convert to an appropriate format.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!