The full-length sequence of TRAF6 was inserted into the pcDNA3.1 plasmid to overexpress TRAF6 in PANC-1 cells (Invitrogen, CA, USA). Cell transfection was performed using Lipofectamine 2000 Reagent (Invitrogen) in line with the manufacturer’s instruction.
Panc 1 cells
Manufactured by Thermo Fisher Scientific
Sourced in United States
PANC-1 cells are a human pancreatic ductal epithelial cell line derived from a pancreatic carcinoma. They are commonly used in research related to pancreatic cancer.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using panc 1 cells
1
TRAF6 Overexpression in PANC-1 Cells
2
Evaluating PANC1 Cell Viability
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PANC1 cells (Invitrogen) were plated in 96-well plates at the density of 8000 cells/well and cultured overnight. On the next day, their medium was replaced with fresh medium supplemented with PNGase F, or the pH of the culture medium was adjusted with hydrochloric acid to 3.5 (100 μl/well). After incubation, 10 μl of the cell counting reagent CCK-8 (Vazyme) was added to each well and the cells were incubated for 2 h at 37 °C in 5% CO2. After incubation, the absorbance value of each well was measured at 450 nm. Three independent experiments were performed.
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