P stat3 9138

Manufactured by Cell Signaling Technology

The P-Stat3 (#9138) is a primary antibody that specifically detects phosphorylated Stat3 (Tyr705). It is designed for use in western blotting applications.

Automatically generated - may contain errors

Lab products found in correlation

E cadherin 3195, by Cell Signaling Technology (1 mentions) Stat3 9132, by Cell Signaling Technology (1 mentions) N cadherin 4061, by Cell Signaling Technology (1 mentions) Hrp conjugated anti rabbit or mouse igg, by Vector Laboratories (1 mentions)

2 protocols using p stat3 9138

Mucosal Protein Profiling via Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blot analysis was carried out with standard methods on the mucosal protein lysates obtained from each mouse. Specific antibodies against p-Stat3 (#9138), Stat3 (#9132), p-Src (#6943), Src (#2123), N-Cadherin (#4061), E-Cadherin (#3195), arginase-1 (#9819), and iNOS (#2982) were purchased from Cell Signaling Technology (Beverly, MA). Anti-FOXP3 antibody (ab54501) was purchased from Abcam (Cambridge, UK) and anti-α-tubulin (sc-20357) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Huang G., Khan I., Li X., Chen L., Leong W., Ho L.T, & Hsiao W.L. (2017). Ginsenosides Rb3 and Rd reduce polyps formation while reinstate the dysbiotic gut microbiota and the intestinal microenvironment in ApcMin/+ mice. Scientific Reports, 7, 12552.

+ Open protocol

+ Expand

Western Blot Analysis of Cellular Signaling

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blot on cell lysates was performed as described.(35 (link)) Cell lysates were examined by routine
western blotting. The blots were incubated with Abs against STAT3 (9139, Cell
Signaling Technology), phosphorylated STAT3 (p-STAT3, 9138, Cell Signaling
Technology), NFκB P65 (8242, Cell Signaling Technology), and
phosphorylated P65 (p-P65, 3033, Cell Signaling Technology), or actin, and
subsequently incubated with HRP-conjugated anti-rabbit or mouse IgG (Vector
Laboratories) in TBS containing 3% BSA (bovine albumin) and
0.05% Tween 20. The final reaction was developed with a chemiluminescent
system (36 (link)).

Meng L., Bai Z., He S., Mochizuki K., Liu Y., Purushe J., Sun H., Wang J., Yagita H., Mineishi S., Fung H., Yanik G.A., Caricchio R., Fan X., Crisalli L.M., Hexner E.O., Reshef R., Zhang Y, & Zhang Y. (2015). The Notch ligand DLL4 defines a capability of human dendritic cells in regulating Th1 and Th17 differentiation. Journal of immunology (Baltimore, Md. : 1950), 196(3), 1070-1080.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!