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P stat3 9138

Manufactured by Cell Signaling Technology

The P-Stat3 (#9138) is a primary antibody that specifically detects phosphorylated Stat3 (Tyr705). It is designed for use in western blotting applications.

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2 protocols using p stat3 9138

1

Mucosal Protein Profiling via Western Blot

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Western blot analysis was carried out with standard methods on the mucosal protein lysates obtained from each mouse. Specific antibodies against p-Stat3 (#9138), Stat3 (#9132), p-Src (#6943), Src (#2123), N-Cadherin (#4061), E-Cadherin (#3195), arginase-1 (#9819), and iNOS (#2982) were purchased from Cell Signaling Technology (Beverly, MA). Anti-FOXP3 antibody (ab54501) was purchased from Abcam (Cambridge, UK) and anti-α-tubulin (sc-20357) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA).
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2

Western Blot Analysis of Cellular Signaling

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Western blot on cell lysates was performed as described.(35 (link)) Cell lysates were examined by routine
western blotting. The blots were incubated with Abs against STAT3 (9139, Cell
Signaling Technology), phosphorylated STAT3 (p-STAT3, 9138, Cell Signaling
Technology), NFκB P65 (8242, Cell Signaling Technology), and
phosphorylated P65 (p-P65, 3033, Cell Signaling Technology), or actin, and
subsequently incubated with HRP-conjugated anti-rabbit or mouse IgG (Vector
Laboratories) in TBS containing 3% BSA (bovine albumin) and
0.05% Tween 20. The final reaction was developed with a chemiluminescent
system (36 (link)).
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