Stock solutions of 600 mg/ml hemoglobin (MP Biomedicals, 02151234), 400 mg/mL PEG-20K (Sigma, 81300), or 400 mg/mL KCl were prepared in PBS. Osmolality was measured using OSMOMAT 30 (Gonotec). Three measurements for each dilution were performed. Data were analysed using GraphPad and fitted with either a straight line or a third order polynomial. All cell media were adjusted to be isoosmotic within each experiment, unless otherwise stated.
Peg 20k
Manufactured by Merck Group
PEG-20K is a polyethylene glycol (PEG) compound with an average molecular weight of 20,000 Daltons. It is a water-soluble polymer commonly used in various laboratory applications.
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Lab products found in correlation
3 protocols using peg 20k
1
Hemoglobin, PEG-20K, and KCl Solutions
2
Synthesis of Octabutoxy Naphthalocyanine
3
Hydrogel Swelling Dynamics and Polymer Interactions
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We use commercially available "aquapearls" (sodium polyacrylate hydrogel particles, Deco-Boulevard, Lohmar, Germany), intended for decorative purposes, as model hydrogel particles. The material is provided as dry spheres with an average radius of R dry ≈ 1.21 mm. Submersed in water, these hydrogel particles can swell up to over 100 times their original volume.
We use polymer solutions of the following macromolecules (from small to large): dextran 70k (from Leuconostoc spp, M w = 70 kDa, Sigma-Aldrich, radius of gyration R g ≈ 6 nm [23] (link)), polyethylene glycol 20k, (PEG 20k, M w = 20 kDa, Sigma-Aldrich, R g ≈ 7 nm [24] (link)), and the bigger polyethylene oxide 200k (PEO 200k, M w = 200 kDa, Sigma-Aldrich, R g ≈ 22 nm [24] (link)). Chemically, PEG 20k and PEO 200k are the same and only differ in molecular weight and hence chain length. For simplicity, we will refer to a "saline solution" when we use a 100 mM NaCl solution (NaCl, Sigma Aldrich, dissolved in MilliQ deionized water). We did not use any other salts or salt concentrations.
We use polymer solutions of the following macromolecules (from small to large): dextran 70k (from Leuconostoc spp, M w = 70 kDa, Sigma-Aldrich, radius of gyration R g ≈ 6 nm [23] (link)), polyethylene glycol 20k, (PEG 20k, M w = 20 kDa, Sigma-Aldrich, R g ≈ 7 nm [24] (link)), and the bigger polyethylene oxide 200k (PEO 200k, M w = 200 kDa, Sigma-Aldrich, R g ≈ 22 nm [24] (link)). Chemically, PEG 20k and PEO 200k are the same and only differ in molecular weight and hence chain length. For simplicity, we will refer to a "saline solution" when we use a 100 mM NaCl solution (NaCl, Sigma Aldrich, dissolved in MilliQ deionized water). We did not use any other salts or salt concentrations.
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