Irdye 800cw conjugated goat anti mouse

Manufactured by LI COR

Sourced in United States

The IRDye 800CW-conjugated goat anti-mouse is a fluorescent-labeled secondary antibody reagent. It is designed to detect and quantify mouse primary antibodies in various applications, such as Western blotting, immunohistochemistry, and cell-based assays.

Automatically generated - may contain errors

Lab products found in correlation

Bca assay, by Thermo Fisher Scientific (3 mentions) Odyssey two color infrared imaging system, by LI COR (2 mentions) Irdye 680rd conjugated goat anti rabbit, by LI COR (2 mentions) Quantity one system, by Bio-Rad (2 mentions) Ripa buffer, by Cell Signaling Technology (2 mentions) Phenyl methyl sulfonyl fluoride (pmsf), by Solarbio (2 mentions) 60 mm dishes, by Thermo Fisher Scientific (1 mentions) Odyssey scanner, by LI COR (1 mentions) Mouse anti β actin, by Merck Group (1 mentions) Rabbit anti gfp, by Thermo Fisher Scientific (1 mentions) Ripa buffer, by Merck Group (1 mentions) Complete protease inhibitor cocktail, by Roche (1 mentions) Rabbit anti phospho erk1 2, by Cell Signaling Technology (1 mentions) Rabbit anti phospho akt, by Cell Signaling Technology (1 mentions) Rabbit anti caspase 3, by Cell Signaling Technology (1 mentions) Rabbit anti bcl 2, by Cell Signaling Technology (1 mentions) Phenylmethylsulfonyl fluoride, by Thermo Fisher Scientific (1 mentions) Protein inhibitor, by Roche (1 mentions) Nbp2 42225, by Novus Biologicals (1 mentions) Mab932, by R&D Systems (1 mentions)

Close spelling variants of this product

IRDye 800CW–conjugated goat anti–mouse and anti–rabbit IgG IRDye 800CW-conjugated goat anti-rabbit IgG or anti-mouse IgG IRDye 800CW-conjugated goat anti-mouse IgG IRDye 800CW-conjugated goat anti-mouse antibody IRDye 800CW-conjugated goat anti-mouse antibody IgG IRDye 800CW dye-conjugated goat anti-mouse Ig IRDye® 800CW Conjugated Goat (polyclonal) Anti-mouse secondary antibody IRDye 800CW-conjugated goat (polyclonal) anti-mouse IgG IRDye®-800CW-conjugated goat anti-mouse IgG (H + L) antibody IRDye 800CW-conjugated goat-anti-mouse secondary antibody

4 protocols using irdye 800cw conjugated goat anti mouse

Immunoblotting of S. cerevisiae and HeLa Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

For immunoblotting of yeast S. cerevisiae whole-cell lysates, cells were cultured and lysates were prepared as previously described in SDS loading buffer^{28 (link)}. Proteins were resolved by SDS-PAGE and blotted using affinity-purified guinea pig anti-Gle1 (ASW 43^{10 (link)}), rabbit anti-GFP (Thermo Fisher Scientific, Waltham, MA), or anti-yPgk1 (Thermo Fisher Scientific). IRDye 800CW-conjugated goat anti-mouse (LI-COR, Lincoln, NE) or Alexa Fluor 700 goat anti-rabbit (Thermo Fisher Scientific) antibodies (1:5000) were visualized with the Li-Cor Odyssey scanner (Lincoln, NE).
For HeLa cell lysate immunoblotting, cells were grown in 60 mm dishes (Fisher Scientific, Pittsburg, PA) and lysed in RIPA buffer (Sigma, St. Louis, MO) supplemented with EDTA-free cOmplete protease inhibitor cocktail (Roche Applied Science, Indianapolis, IN). Proteins were resolved by SDS-PAGE and blotted with rat anti-mCherry (Sigma), anti-hNup42/NUPL2 (Sigma), mouse anti-beta-actin (Sigma), or anti-hGle1^{17 (link)} antibodies. Infrared 700- or 800-conjugated secondary antibodies (Thermo Fisher Scientific) were visualized with the Li-Cor Odyssey scanner.

Adams R.L., Mason A.C., Glass L., A.d.i.t.i, & Wente S.R. (2017). Nup42 and IP6 coordinate Gle1 stimulation of Dbp5/DDX19B for mRNA export in yeast and human cells. Traffic (Copenhagen, Denmark), 18(12), 776-790.

+ Open protocol

+ Expand

Western Blot Analysis of Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Proteins were extracted from tissues using the RIPA buffer (Cell Signaling Technology, USA) containing 1 mM NaF and 1 mM phenylmethylsulfonyl fluoride (PMSF; Solarbio, China). Protein concentrations were determined using the BCA assay (Thermo Scientific, USA). Equivalent amounts of protein samples were separated by 10% SDS-PAGE and transferred to nitrocellulose membranes. The primary antibodies used were rabbit anti-phospho-Akt (cat#4060s), rabbit anti-phospho-ERK1/2 (cat#4370s), rabbit anti-caspase-3 (cat#9664) and rabbit anti-Bcl-2 (cat#2870) (all from Cell Signaling Technology, USA; used at 1:1000 dilution) or the mouse anti-β-actin (TA09, ZS-Bio, China; used at 1:2000). The secondary antibodies used were IRDye 680RD-conjugated goat anti-rabbit (cat#926-32220) or IRDye 800CW-conjugated goat anti-mouse (cat#926-32211) antibodies (LI-COR, USA; used at 1:10,000). The band density was scanned using the Odyssey Two-Color Infrared Imaging System (LI-COR, USA) and quantified using the Quantity One System (Bio-Rad, USA).

Zhou Y., Hu Q., Chen F., Zhang J., Guo J., Wang H., Gu J., Ma L, & Ho G. (2015). Human umbilical cord matrix-derived stem cells exert trophic effects on β-cell survival in diabetic rats and isolated islets. Disease Models & Mechanisms, 8(12), 1625-1633.

+ Open protocol

+ Expand

Western Blot Analysis of Extracellular Vesicles

Check if the same lab product or an alternative is used in the 5 most similar protocols

The collected EVs were lysed in Pierce immunoprecipitation lysis buffer containing 1× protein inhibitor (Roche) and 1 mM phenylmethylsulfonyl fluoride (Thermo Fisher Scientific), and the protein concentration was quantified using the bicinchoninic acid assay (BCA assay; Thermo Fisher Scientific). Protein lysates were resolved by SDS–polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride (PVDF) membranes (Invitrogen). The PVDF membrane was blocked with 5% nonfat dry milk in tris-buffered saline buffer for 1 hour at room temperature (RT) and then immunoblotted with 500-fold diluted primary antibodies overnight at 4°C. The primary antibodies used in this study included mouse anti-human MCSP (R&D Systems, MAB2585), MCAM (R&D Systems, MAB932), ErbB3 (R&D Systems, MAB3482), LNGFR (R&D Systems, MAB367; or Santa Cruz Biotechnology, sc-271708), calnexin (Abcam, ab112995), and CD63 (Novus, NBP2-42225). Proteins were analyzed under denaturing and reducing/nonreducing conditions according to the manufacturer’s instructions. After incubation, the PVDF membrane was washed with phosphate-buffered saline (PBS) containing 0.1% Tween 20 and then incubated with IRDye 800CW–conjugated goat anti-mouse (LI-COR, 926-32210, 10,000-fold dilution) for 1 hour at RT. After washing, protein bands were detected using the Odyssey LI-COR CLx Imaging System.

Wang J., Wuethrich A., Sina A.A., Lane R.E., Lin L.L., Wang Y., Cebon J., Behren A, & Trau M. (2020). Tracking extracellular vesicle phenotypic changes enables treatment monitoring in melanoma. Science Advances, 6(9), eaax3223.

+ Open protocol

+ Expand

Western Blot Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Proteins were extracted from cultured cells using RIPA buffer (CellSignalingTechnology) containing1 mMNaFand1 mM PMSF (Solarbio, Beijing, China). Protein concentrations were determined using the BCA assay (Thermo Scientific, Waltham, MA, USA). Equivalent amounts of protein samples were separated by 10% SDS-PAGE and transferred to nitrocellulose membranes (Whatman, Dusseldorf, Germany). The membranes were incubated with 5% BSA in TBST at room temperature to block nonspecific binding and then incubated with primary antibodies of rabbit antiphospho-AMPKa (Thr172) or rabbit anti-phospho-ERK1/2 (Thr202/Tyr204) (1:1000, Cell Signaling Technology), or mouse anti-b-actin (1:2000, ZS-Bio) overnight at 4 8C. Subsequently, the membranes were incubated with secondary antibodies (IRDye 680RD-conjugated goat anti-rabbit or IRDye 800CW-conjugated goat anti-mouse (1:10 000, LI-COR, USA)) for 1 h at room temperature. The band density was visualized, scanned, and analyzed using the Odyssey Two-Color Infrared Imaging System (LI-COR) and quantified using the Quantity OneSystem (Bio-Rad).

Zhang J., Zhou Y., Chen C., Yu F., Wang Y., Gu J., Ma L, & Ho G. (2015). ERK1/2 mediates glucose-regulated POMC gene expression in hypothalamic neurons. Journal of molecular endocrinology, 54(2).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!