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2 deoxycytidine 5 monophosphate

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2'-deoxycytidine-5'-monophosphate is a nucleotide compound that serves as a building block for DNA synthesis and modification. It consists of the nucleoside 2'-deoxycytidine linked to a phosphate group at the 5' position. This compound is commonly used in biochemical research and applications involving DNA-related processes.

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Lab products found in correlation

2 deoxyguanosine 5 monophosphate, by Merck Group (3 mentions) 2 deoxyadenosine 5 monophopshate, by Merck Group (2 mentions) 2 deoxythmidine 5 monophosphate, by Merck Group (2 mentions) 1n hydrochloric acid, by Merck Group (2 mentions) 2 deoxyadenosine 5 monophosphate, by Merck Group (2 mentions) Uridine 5 monophosphate, by Merck Group (1 mentions) Cytidine 5 monophosphate, by Merck Group (1 mentions) 1n sodium hydroxide, by Merck Group (1 mentions) Sodium chloride (nacl), by Merck Group (1 mentions) Deoxyribonucleic acid sodium salt, by Merck Group (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions) 9 10 anthracenediyl bis methylene dimalonic acid abda, by Merck Group (1 mentions) 2 hydroxy 2 methylpropiophenone, by Merck Group (1 mentions) Si wafers, by Merck Group (1 mentions) Poly ethylene glycol diacrylate pegda, by Merck Group (1 mentions) Mf 319 developer, by MicroChem (1 mentions) Tris borate edta, by Merck Group (1 mentions) S1813 photoresist, by MicroChem (1 mentions) Hydrofluoric acid hf, by Merck Group (1 mentions) Tris edta, by Merck Group (1 mentions)

5 protocols using 2 deoxycytidine 5 monophosphate

1

Nucleotide Metabolism Assay Protocol

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The list of the ANPs and chemotherapeutics used in this study is provided in Tables S1 and Figure S9. The source of other chemical reagents was as follows: Uridine (Urd) and adenosine-5′-triphosphate (ATP) Calbiochem; cytidine (Cyd), Valeant Pharmaceuticals; cytidine-5′-monophosphate (CMP), uridine-5′-monophosphate (UMP), 2′-deoxycytidine-5′-monophosphate (dCMP) and 2′-deoxyuridine-5′-monophosphate (dUMP), Sigma-Aldrich; arabinocytidine-5′-monophosphate (araCMP), [5-3H]-radiolabeled CDV and [5-3H]-uridine, Moravek Biochemicals; and [5-3H]-radiolabeled cytidine, MP Biochemicals.
Topalis D., Nogueira T.C., De Schutter T., El Amri C., Krečmerová M., Naesens L., Balzarini J., Andrei G, & Snoeck R. (2016). Resistance to the nucleotide analogue cidofovir in HPV(+) cells: a multifactorial process involving UMP/CMP kinase 1. Oncotarget, 7(9), 10386-10401.
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2

Nucleotide Synthesis and Characterization

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2′-deoxycytidine-5′-monophosphate
(dCMP), 2′-deoxyguanosine-5′-monophosphate (dGMP), 2′-deoxyadenosine-5′-monophopshate
(dAMP), 2′-deoxythmidine-5′-monophosphate (dTMP), sodium
chloride, 1N hydrochloric acid, and 1N sodium hydroxide were purchased
from Sigma-Aldrich. Anatase TiO2 particles were purchased
from US Research Nanomaterials stock number #US3498. All chemicals
were used without additional modification or purification. For clarity,
nucleotides have three main functional groups, the nitrogenous ring,
the ribose sugar ring, and the phosphate group. The nitrogenous ring
and ribose are defined as the nucleosides. The nucleoside with the
phosphate group is the nucleotide.
Sit I., Quirk E., Hettiarachchi E, & Grassian V.H. (2022). Differential Surface Interactions and Surface Templating of Nucleotides (dGMP, dCMP, dAMP, and dTMP) on Oxide Particle Surfaces. Langmuir, 38(49), 15038-15049.
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3

Analyzing Nucleic Acid Components

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Adenine, guanine, cytosine, thymine, adenosine, 2′-deoxyadenosine 5′-monophosphate, 2′-deoxyguanosine 5′-monophosphate, 2′-deoxycytidine 5′-monophosphate, 2′-deoxythymidine 5′-monophosphate, adenosine 5′-diphosphate, deoxyadenosine 5′-triphosphate, deoxyribonucleic acid sodium salt from salmon testes (approximately 2000 bp), 9,10-anthracenediyl-bis(methylene)dimalonic acid (ABDA) and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich. Deionized (DI) water with a specific resistance of 18.2 MΩ was used.
Zheng X.T., Lai Y.C, & Tan Y.N. (2019). Nucleotide-derived theranostic nanodots with intrinsic fluorescence and singlet oxygen generation for bioimaging and photodynamic therapy. Nanoscale Advances, 1(6), 2250-2257.
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4

Fabrication of Microfluidic Devices

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Chemicals and materials were obtained from the following sources and used without further purification: 2'-Deoxyadenosine-5'-monophosphate (Alfa Aesar); Thymidine-5'-monophosphate disodium salt (Alfa Aesar); 2'-Deoxycytidine-5'-monophosphate (Alfa Aesar); 2'-Deoxyguanosine-5'-monophosphate disodium salt hydrate (Alfa Aesar); potassium chloride (1 and 3 m, Sigma-Aldrich); Tris-EDTA (100×, Sigma-Aldrich); Tris-Borate-EDTA (10×, Sigma-Aldrich); silver (Ag) wires (>99%, Sigma-Aldrich); S1813 photoresist (MicroChem); MF319 developer (MicroChem); potassium hydroxide (KOH) pellets (Fisher Scientific); hydrofluoric acid (HF) (Sigma-Aldrich); Fluorolink MD 700 (Solvay); 2-hydroxy-2-methylpropiophenone (Sigma-Aldrich); NOA72 (Norland Products); poly(ethylene glycol) diacrylate (PEGDA) (Mn = 250, Sigma-Aldrich); 2,2-dimethoxy-2-phenylacetophenone (Sigma-Aldrich); Si wafers (P/B, resistivity 5-10 Ωcm, orientation of (100), and 525 ± 25 μm thickness) (WaferPro); polyethylene terephthalate (PET) with 250 μm thickness (Goodfellow); polymethylmethacrylate (PMMA) with a thickness of 250 μm (Goodfellow); polymethylmethacrylate (PMMA) of 1 mm thickness (ePlastics).
Choi J., Jia Z., Riahipour R., McKinney C.J., Amarasekara C.A., Weerakoon-Ratnayake K.M., Soper S.A, & Park S. (2021). Label Free Identification of Single Mononucleotides by Nanoscale Electrophoresis. Small (Weinheim an der Bergstrasse, Germany), 17(42), e2102567.
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5

Nucleotide Zeta Potential Measurement

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2′-deoxyadenosine-5′-monophopshate (dAMP), 2′-deoxyguanosine-5′-monophosphate (dGMP), 2′-deoxycytidine-5′-monophosphate (dCMP), 2′-deoxythmidine-5′-monophosphate (dTMP), sodium chloride, 1N hydrochloric acid, and 1N sodium hydroxide were purchase from Sigma-Aldrich (where 1N means 1 normal concentrations of these solutions). Hematite and goethite particles were purchased from Alfa Aesar, MA. Zeta potential measurements of nucleotides at pH 5 were performed with the Zetasizer Nano from Malvern Instruments. Triplicate measurements are reported with 1σ standard deviation for the indicated error.
Sit I., Young M.A., Kubicki J.D, & Grassian V.H. (2023). Distinguishing different surface interactions for nucleotides adsorbed onto hematite and goethite particle surfaces through ATR-FTIR spectroscopy and DFT calculations. Physical Chemistry Chemical Physics, 25(30), 20557-20566.
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