Pltp activity assay kit

Manufactured by Merck Group

The PLTP Activity Assay Kit is a laboratory tool used to measure the activity of phospholipid transfer protein (PLTP) in biological samples. PLTP is an enzyme involved in the transfer of phospholipids between lipoproteins, and this kit provides a quantitative method to assess its activity levels.

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Lipase activity assay kit 3, by Merck Group (1 mentions)

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Activity Assay Kits

2 protocols using pltp activity assay kit

Serum Enzyme Activity Assays

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Serum enzyme activities were determined using a commercially available kit. CETP enzyme activity was assayed using a cell-free assay according to the manufacturer’s protocol (Roar Biomedical).
LCAT activity assays were performed with an LCAT Activity Assay Kit (Fluorometric) from Cell Biolabs (Cat# STA-615). Whole-serum lipase activity was evaluated using Lipase Activity Assay Kit III from Sigma-Aldrich (Cat# MAK048), and PLTP activity assays were performed using PLTP Activity Assay Kit from Sigma-Aldrich (Cat# MAK108).

Zhu L., Luu T., Emfinger C.H., Parks B.A., Shi J., Trefts E., Zeng F., Kuklenyik Z., Harris R.C., Wasserman D.H., Fazio S, & Stafford J.M. (2018). CETP Inhibition Improves HDL Function but Leads to Fatty Liver and Insulin Resistance in CETP-Expressing Transgenic Mice on a High-Fat Diet. Diabetes, 67(12), 2494-2506.

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PLTP Activity Assay Protocol

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Cells were seeded at a density of 2 to 5 × 10⁵ cells/well in 6-well plates and grown overnight at 37 °C. The following day, cells were washed with PBS and incubated in NP-40 lysis buffer for 20 min at 4 °C. The protein lysates were diluted (1:500–1:5000) and used in enzymatic activity assays following the instructions in the PLTP Activity Assay Kit (Sigma Aldrich, MAK108) in 96-well opaque-bottom plates. The activity levels of the samples were measured using a Gen5 (Biotek) microplate reader.

Gnanapradeepan K., Indeglia A., Stieg D.C., Clarke N., Shao C., Dougherty J.F., Murali N, & Murphy M.E. (2022). PLTP is a p53 target gene with roles in cancer growth suppression and ferroptosis. The Journal of Biological Chemistry, 298(12), 102637.

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