Egm 2 singlequots growth factors

Poly (ethylene) glycol diacrylate 10,000 kDa (PEGDA) was procured from Laysan Bio Inc. (Alabama). Gelatin from porcine skin, photo-initiator (2-Hydroxy-4’-(2-hydroxyethoxy)-2-methylpropiophenone), methacrylic anhydride, ethylene glycol, and bovine serum albumin (BSA) were obtained from Sigma-Aldrich (St. Louis, MO). Geltrex™ LDEV-free reduced growth factor basement membrane, Dulbecco’s Phosphate Buffer Saline (DPBS), Dulbecco’s Modified Eagle Medium (DMEM), Penicillin-Streptomycin (Pen Strep), 0.25% Trypsin-EDTA, Fetal Bovine Serum (FBS), and collagenase Type I were acquired from Gibco by Life Technologies (Grand Island, NY). Hoechst 33342, Alexa Fluor™ 488 phalloidin, methanol, acetone, and Slide-A-Lyzer Dialysis Cassette G2 (10k MWCO) were obtained from Thermofisher Scientific (Waltham, MA). XTT Proliferation Assay kit was purchased from ATCC (Manassas, VA). Endothelial cell basal medium-2 (EBM-2) and EGM-2 SingleQuots growth factors were obtained from Lonza (Walkersville, MD).

Rat tail high concentration collagen type I was obtained from Corning (Bedford, MA). Sodium hydroxide, fluorescein isothiocyanate-dextran of 150K MW (FITC-dextran), sodium acetate, cysteine hydrochloride, sodium phosphate monobasic, sodium phosphate dibasic, papain suspension, cytochalasin D, and (–)-blebbistatin were procured from Sigma Aldrich (St. Louis, MO). Y-27632 and Na₂EDTA were purchased from EMD Millipore (Kankakee, IL). Dulbecco’s Phosphate Buffer Saline (DPBS), RPMI Medium 1640, Penicillin-Streptomycin (Pen Strep), 0.25% Trypsin-EDTA, fetal bovine serum (FBS), and collagenase Type I were acquired from Gibco by Life Technologies (Grand Island, NY). D-(–)-Ribose, Hoechst 33342, and Alexa Fluor™ 488 phalloidin were obtained from Thermofisher Scientific (Waltham, MA). AlamarBlue™ cell viability reagent was purchased from Invitrogen by Thermo Fisher Scientific (Eugene, OR). Acetone, methanol, and glacial acetic acid were acquired from Fisher Chemical (New Jersey). Endothelial cell basal medium-2 (EBM-2) and EGM-2 SingleQuots growth factors were obtained from Lonza (Walkersville, MD).