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Irdye 800cw dye conjugated goat anti mouse ig

Manufactured by LI COR
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IRDye 800CW dye-conjugated goat anti-mouse Ig is a near-infrared fluorescent dye-labeled secondary antibody. It is designed for detection and quantification of mouse immunoglobulins in Western blotting, ELISA, and other immunoassay applications.

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Lab products found in correlation

Mouse monoclonal anti v5 antibody, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

IRDye 800CW-conjugated goat anti-mouse IRDye 800CW goat anti-mouse Anti-goat IRDye 800CW Anti-mouse IRDye 800CW Goat anti-mouse 800CW IRDye goat anti-mouse IRDye 800CW

2 protocols using irdye 800cw dye conjugated goat anti mouse ig

1

Western Blot Detection of Tagged Proteins

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Cell lysates were generated, and proteins resolved by SDS-PAGE and transferred to nitrocellulose as described [19 (link)]. V5-tagged proteins were detected with mouse monoclonal anti-V5 antibody (ThermoFisher) at 0.3 μg/ml. HA-tagged proteins were detected with mouse (Covance) or rat (Roche) monoclonal anti-V5 antibodies at 1 μg/ml or 12.5 ng/ml respectively. Vacuolar phosphatase [20 (link)] was detected with anti-VTC at 1:5000. Bound antibodies were revealed with either IRDye 800CW dye-conjugated goat anti-mouse Ig and IRDye680RD dye-conjugated goat anti-rabbit Ig (LI-COR) at 25 ng/ml and data was visualized on a LI-COR Odyssey.
Jensen B.C., Vaney P., Flaspohler J., Coppens I, & Parsons M. (2021). Unusual features and localization of the membrane kinome of Trypanosoma brucei. PLoS ONE, 16(10), e0258814.
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2

Western Blot Detection of Tagged Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Parasite lysates were generated, and proteins (5×106 cell equivalents) were resolved by SDS-PAGE and transferred to nitrocellulose as described (Jensen et al., 2003 (link)). V5-tagged proteins were detected with mouse monoclonal anti-V5 antibody clone SV5-Pk1 (ThermoFisher) at 0.3 μg/ml. HA-tagged proteins were detected with either mouse clone 16B12 (Biolegend) or rat clone 3F10 (Roche) monoclonal anti-HA antibodies at 1 μg/ml or 12.5 ng/ml respectively. Rabbit polyclonal antibodies to either phosphoglycerate kinase (PGK) (Parker et al., 1995 (link)) or CK2α (Jensen et al., 2007 (link)) were used at 1:10,000 and 1:5000 respectively. Bound antibodies were revealed with either IRDye 680 dye-conjuguated goat anti-rabbit Ig (Li-COR) or IRDye 800CW dye-conjugated goat anti-mouse Ig (Li-COR) at 25 ng/ml and data was visualized on a Li-COR Odyssey.
Parsons M., Parsons B., Dean M., DeRocher A.E., Islam Z., Maly D.J, & Jensen B.C. (2023). An essential Trypanosoma brucei protein kinase: a functional analysis of regulation and the identification of inhibitors. Frontiers in parasitology, 2, 1272378.
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