system (DSC-60 Plus, Shimadzu, Co., Japan) according to Wang et al.43 (link) Gluten protein was freeze-dried and ground through
120-mesh sieve. Gluten samples (3.0–5.0 mg) were tableted into
an aluminum pan (S201-52943, Shimadzu, Japan) and then heated to 250
°C with constant nitrogen purging at a constant rate of 5 °C/min.
A sealed empty aluminum pan was used as a reference. The enthalpy
change (ΔH) and peak temperature (Tp) of gluten protein with addition of different amounts
of KGM were obtained using TA-60 Analysis software (version 2.21,
Shimadzu Co., Japan).