Pe conjugated anti ly6g

Manufactured by Thermo Fisher Scientific

Sourced in United States

PE)-conjugated anti-Ly6G is a lab equipment product that functions as a cell surface marker. It is used to identify and isolate Ly6G-positive cells in flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

3 protocols using pe conjugated anti ly6g

Analyzing Placental and Splenic Immune Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

To analyze the populations of inflammatory cells within the placental tissue and spleen of pregnant mice, the mice were sacrificed under general anesthesia. Placental and splenic cells were
isolated from mice, as described previously [30 (link), 31 (link)]. The cells were washed, treated with erythrocyte lysis
buffer, and resuspended in PBS supplemented with 3% fetal bovine serum for flow cytometry analysis. Cells were labeled with the following antibodies purchased from eBioscience (San Diego,
CA, USA): phycoerythrin (PE)-conjugated anti-Ly6G (Cat 12-9668-82), PE-conjugated anti-CD11b (Cat 12-0112-82), FITC-conjugated anti-F4/80 (Cat 11-4801-82), and allophycocyanin-conjugated
anti-CD45 (Cat 17-0451-82). The cells were examined by flow cytometry using a NovoCyte flow cytometer (ACEA Biosciences Inc., San Diego, CA, USA).

HIRATA Y., KATSUKURA Y., HENMI Y., OZAWA R., SHIMAZAKI S., KUROSAWA A., TORII Y., TAKAHASHI H., IWATA H., KUWAYAMA T, & SHIRASUNA K. (2021). Advanced maternal age induces fetal growth restriction through decreased placental inflammatory cytokine expression and immune cell accumulation in mice. The Journal of Reproduction and Development, 67(4), 257-264.

+ Open protocol

+ Expand

Analyzing Inflammatory Cells in Placental Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

To analyze the inflammatory cells within placental tissue of pregnant mice, five placentas from each pregnant mouse were used, as previously described [14 (link)]. Briefly, mice were sacrificed under general anesthesia. Placental cells were isolated from mice as described previously [14 (link)]. The placental cells were washed, treated with erythrocyte lysis buffer, and resuspended in PBS supplemented with 3% FBS for flow cytometry analysis. Cells were
labeled with the following antibodies purchased from eBioscience (San Diego, CA, USA): phycoerythrin (PE)-conjugated anti-Ly6G, PE-conjugated anti-CD11b, and
allophycocyanin-conjugated anti-CD45. The cells were examined by flow cytometry (NovoCyte flowcytmeter; ACEA Biosciences, San Diego, CA, USA). Isotype control antibodies were used
as negative controls to exclude nonspecific background staining.

SANO M., SHIMAZAKI S., KANEKO Y., KARASAWA T., TAKAHASHI M., OHKUCHI A., TAKAHASHI H., KUROSAWA A., TORII Y., IWATA H., KUWAYAMA T, & SHIRASUNA K. (2020). Palmitic acid activates NLRP3 inflammasome and induces placental inflammation during pregnancy in mice. The Journal of Reproduction and Development, 66(3), 241-248.

+ Open protocol

+ Expand

Flow Cytometry Analysis of Immune Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols

LP cells from colon or splenic immune cells were sorted using a FACS flow cytometer (BD Biosciences, San Jose, CA) and analyzed using FlowJo_V10 (Treestar, Ashlan, OR). The gating strategy has been shown in supplementary Figure 8. For macrophage and neutrophil analyses, cells were incubated with the following antibodies: FITC-conjugated anti-CD11b (11-0112-82, eBioscience, San Diego, CA), APC-conjugated anti-CD11c (17-0114-81, eBioscience), PE-cyanine7-conjugated anti-F4/80 (25-4801-82, eBioscience), PE-conjugated anti-CD206 (12-2061-80, eBioscience), and PE-conjugated anti-Ly6G (12-5931-81, eBioscience); for T cell analyses, cells were incubated with the following antibodies: APC-conjugated anti-CD3 (17-0032-82, eBioscience), FITC-conjugated anti-CD4 (11-0042-81, eBioscience), and PE-conjugated anti-CD8 (12-0081-81, eBioscience); for B cell and NK cell analyses, cells were incubated with the following antibodies: FITC-conjugated anti-CD19 (11-0193-81, eBioscience), and PE-conjugated anti-NK1.1 (12-5941-81, eBioscience).

Song L., Chang R., Sun X., Lu L., Gao H., Lu H., Lin R., Xu X., Liu Z, & Zhan L. (2021). Macrophage-derived EDA-A2 inhibits intestinal stem cells by targeting miR-494/EDA2R/β-catenin signaling in mice. Communications Biology, 4, 213.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!