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F chemibis 3

Manufactured by DNR Bio-Imaging Systems
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The F-ChemiBIS 3.2 is a high-performance imaging system designed for chemiluminescence and fluorescence detection. It features a cooled CCD camera, adjustable lens, and multiple illumination options, enabling sensitive and versatile imaging capabilities.

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Lab products found in correlation

Pageruler prestained protein ladder, by Thermo Fisher Scientific (2 mentions) Pierce ecl western blotting substrate, by Thermo Fisher Scientific (2 mentions) Amersham ecl select western blotting detection reagent, by GE Healthcare (2 mentions) Anti actin, by Merck Group (2 mentions) Anti iκbα, by Santa Cruz Biotechnology (2 mentions) Coomassie protein assay kit, by Thermo Fisher Scientific (2 mentions) Nupage novex 4 12 bis tris gel, by Thermo Fisher Scientific (2 mentions) Westran clear signal pvdf membranes, by Cytiva (2 mentions) Whatman, by Cytiva (2 mentions) Amersham ecl, by Cytiva (2 mentions)

2 protocols using f chemibis 3

1

Western Blot Analysis of Protein Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein concentration of cell lysates and organs were determined with a Coomassie Protein Assay kit (Thermo Scientific). Proteins were analyzed by SDS-Page using NuPAGE® Novex 4-12% Bis-Tris Gels (Life Technologies), Westran® Clear signal PVDF membranes (Whatman) and the following antibodies: anti-Zbp1 (kindly provided by the Superti-Furga laboratory), anti-Setdb2 (clone 7H7F11, described in this study), anti-IκBα (Santa Cruz Biotechnology sc-371 clone C-21, respectively, Cell Signaling n.4814 clone L35A5) and anti-actin (Sigma #A2066). The protein size was determined with the PageRuler™ Prestained Protein Ladder (Thermo Scientific). Detection was done with Pierce ECL Western blotting substrate (Thermo Scientific) or Amersham ECL select Western blotting detection reagent (GE Healthcare Life Sciences). Gels were visualized with the chemiluminescent gel documentation system F-ChemiBIS 3.2 (DNR Bio-Imaging Systems).
Schliehe C., Flynn E.K., Vilagos B., Richson U., Swaminanthan S., Bosnjak B., Bauer L., Kandasamy R.K., Griesshammer I.M., Kosack L., Schmitz F., Litvak V., Sissons J., Lercher A., Bhattacharya A., Khamina K., Trivett A.L., Tessarollo L., Mesteri I., Hladik A., Merkler D., Kubicek S., Knapp S., Epstein M.M., Symer D.E., Aderem A, & Bergthaler A. (2014). The methyltransferase Setdb2 mediates virus-induced susceptibility to bacterial superinfection. Nature immunology, 16(1), 67-74.
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2

Western Blot Analysis of Protein Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein concentration of cell lysates and organs were determined with a Coomassie Protein Assay kit (Thermo Scientific). Proteins were analyzed by SDS-Page using NuPAGE® Novex 4-12% Bis-Tris Gels (Life Technologies), Westran® Clear signal PVDF membranes (Whatman) and the following antibodies: anti-Zbp1 (kindly provided by the Superti-Furga laboratory), anti-Setdb2 (clone 7H7F11, described in this study), anti-IκBα (Santa Cruz Biotechnology sc-371 clone C-21, respectively, Cell Signaling n.4814 clone L35A5) and anti-actin (Sigma #A2066). The protein size was determined with the PageRuler™ Prestained Protein Ladder (Thermo Scientific). Detection was done with Pierce ECL Western blotting substrate (Thermo Scientific) or Amersham ECL select Western blotting detection reagent (GE Healthcare Life Sciences). Gels were visualized with the chemiluminescent gel documentation system F-ChemiBIS 3.2 (DNR Bio-Imaging Systems).
Schliehe C., Flynn E.K., Vilagos B., Richson U., Swaminanthan S., Bosnjak B., Bauer L., Kandasamy R.K., Griesshammer I.M., Kosack L., Schmitz F., Litvak V., Sissons J., Lercher A., Bhattacharya A., Khamina K., Trivett A.L., Tessarollo L., Mesteri I., Hladik A., Merkler D., Kubicek S., Knapp S., Epstein M.M., Symer D.E., Aderem A, & Bergthaler A. (2014). The methyltransferase Setdb2 mediates virus-induced susceptibility to bacterial superinfection. Nature immunology, 16(1), 67-74.
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