Hca204

Example 4

FIG. 10 shows a non-limiting example of an interior of a device, which is included in embodiments of the present invention. Samples flow from A to B. Test strips for lateral flow assay are composed of (in this order): a sample pad (LF1, GE Healthcare); a second pad (ReliaFlow, Ahlstrom) containing latex-labeled goat anti-human IgG; a membrane (Hi-Flow HF075, Millipore) where both a control antibody (rabbit anti-goat IgG, Thermo Fisher) and a chimeric antibody (chimeric-adalimumab) were immobilized, the latter being composed of the adalimumab variable region fused with mouse IgG2a constant region (Anti-hTNF-α-mlg2a, InvivoGen); an absorbent pad (cellulose fiber, Millipore). 1, no anti-adalimumab antibody in the buffer sample (10 mM phosphate buffer pH 7.4, plus 137 mM NaCl, plus 0.02% triton-X-100); 2, 5 μl of sheep blood; 3, 1 μl of anti-adalimumab antibody (HCA204, 500 m/ml, AbD Serotec) spiked on 5 μl of sheep blood; 4, 1 μl of anti-adalimumab antibody (HCA204, 500 m/ml, AbD Serotec) spiked on 5 μl of sheep blood. Following sample application, 50 μl of buffer (10 mM phosphate buffer pH 7.4, plus 137 mM NaCl, plus 0.02% triton-X-100) was added downstream of the sample application in an angle, and contrary to the direction of sample flow, to create a reflux. Sheep blood was obtained from Rockland Inc. (Limerick, Pa.).