Endometrial organoids were microinjected using an Eppendorf FemtoJet 4x coupled with a Stereo Microscope (Nikon). C. trachomatis CTL2 was diluted in PBS to a final concentration of 5 × 105 – 5 × 106 IFU/mL, vortexed for 30 s, and ~ 5 μL was pipetted into a glass capillary (Sutter Instrument Company) using a microloader tip (Eppendorf). The glass capillary tip was broken off and the organoids were punctured once using a steep vertical angle and injected with equal volumes. When co-injected with fluorescent dextran, C. trachomatis CTL2 was diluted in PBS containing 0.01 mg/mL 3kD Texas-Red dextran (Invitrogen). Live imaging was performed on the Nikon Ti2 described below using a humidified Okolab environmental chamber set to 37°C.
3kd texas red dextran
Manufactured by Thermo Fisher Scientific
The 3kD Texas-Red dextran is a fluorescently-labeled dextran molecule with a molecular weight of 3 kilodaltons. It can be used as a tracer or marker in various biological applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 3kd texas red dextran
1
Microinjection of Endometrial Organoids with C. trachomatis
2
Organoid Microinjection with Chlamydia
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Microinjections were performed using an Eppendorf FemtoJet 4x coupled with a Stereo Microscope (Nikon). Chlamydia strains were diluted in PBS to a final concentration of 5E 5 -5E 6 IFU, vortexed for 30s, and pipetted into a glass needle. Organoids were punctured once using a steep vertical angle and manually injected with equal volumes. When organoids were injected alone or co-injected with 3kD Texas-Red dextran (Invitrogen), dextran was used at a final concentration of 0.01 mg/mL.
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