Dopamine hydrochloride was purchased from McLean (Shanghai, China). Cucurbitacin B was provided by Chengdu Biopurify Phytochemicals Ltd. (Chengdu, China). 4′,6-Diamidino-2-phenylindole (DAPI), RIPA lysis buffer, BCA assay kits, Rhodamine B and annexin V-FITC/PI cell apoptosis kit were obtained from Solarbio (Beijing, China). DCFH-DA and Cell Counting Kit-8 (CCK-8) were purchased from Beyotime Institute of Biotechnology (China). Foetal bovine serum (FBS), RPMI modified medium, trypsin-EDTA and penicillin-streptomycin were purchased from HyClone. All antibodies were obtained from ABclonal.
Annexin 5 fitc pi cell apoptosis kit
Manufactured by Solarbio
Sourced in China
The Annexin V-FITC/PI cell apoptosis kit is a laboratory reagent used to detect and quantify apoptosis, a type of programmed cell death, in cellular samples. The kit contains Annexin V-FITC, a fluorescently-labeled protein that binds to phosphatidylserine, and propidium iodide (PI), a DNA-binding dye. This combination enables the identification of cells undergoing early and late stages of apoptosis.
Automatically generated - may contain errors
Lab products found in correlation
Cell counting kit 8 cck 8, by Beyotime (1 mentions)
Bca assay kit, by Solarbio (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions)
Dcfh da, by Beyotime (1 mentions)
Ripa lysis buffer, by Solarbio (1 mentions)
Rhodamine b, by Solarbio (1 mentions)
Bricyte e6, by Mindray (1 mentions)
3 protocols using annexin 5 fitc pi cell apoptosis kit
1
Cucurbitacin B-Induced Apoptosis in Cells
2
Annexin V-FITC/PI Cell Apoptosis Assay
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The cells were suspended in 100 μL PBS at a concentration of over 1 × 107 cells/mL. Annexin V-FITC/PI cell apoptosis kit (Solarbio Co., Beijing, China) was used to stain the cells for 20 min at room temperature in the dark. After washing and resuspension, the cell apoptosis was detected with a flow cytometer (Bricyte E6, Mindray Bio-Medical Electronics Co., Ltd, Shenzhen, China).
3
Apoptosis Assay for SiHa and HeLa Cells
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Forty-eight-hour post-transfection, SiHa and Hela cells were seeded into 6-well plates at 37°C and 5% CO2 After 12 h incubation, the cells were harvested by trypsinization without EDTA and washed with precooled PBS. Double staining was conducted by Annexin V-FITC/PI cell apoptosis kit (Solarbio, China) and propidium iodide (PI) following the protocol. Then, the cells were analyzed by MoFlo Astrios EQ flow cytometry (Beckman) for the percentage of apoptotic cells. All assay of the samples were in triplicate.
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