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Elisa max deluxe set mouse total ige

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The ELISA MAX Deluxe set mouse total IgE is a laboratory equipment product designed for the quantitative measurement of total IgE levels in mouse samples. It provides the necessary components for performing an enzyme-linked immunosorbent assay (ELISA) to detect and quantify total IgE concentrations.

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Lab products found in correlation

Halt protease and phosphatase inhibitor, by Thermo Fisher Scientific (2 mentions) T per tissue protein extraction reagent, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

ELISA MAX™ Deluxe Set Mouse IgE MAX™ Deluxe Sets ELISA MAX Deluxe ELISA MAX sets Total IgE ELISAs

2 protocols using elisa max deluxe set mouse total ige

1

Quantifying Mouse Lung IgE Levels

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The left lung was collected and homogenized in T-PER tissue protein extraction reagent (Thermo Scientific) supplemented with Halt protease and phosphatase inhibitors (Thermo Scientific) to extract total protein. ELISA MAX Deluxe set mouse total IgE (BioLegend, San Diego CA) was used to quantify IgE levels in both serum and lung tissue homogenate. Lung tissue homogenate immunoglobulin concentrations were normalized to the total protein levels in the sample, quantitated by BCA assay (Pierce, Springfield Township NJ).
Borbet T.C., Pawline M.B., Zhang X., Wipperman M.F., Reuter S., Maher T., Li J., Iizumi T., Gao Z., Daniele M., Taube C., Koralov S.B., Müller A, & Blaser M.J. (2022). Influence of the early-life gut microbiota on the immune responses to an inhaled allergen. Mucosal immunology, 15(5), 1000-1011.
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2

Quantifying Mouse Lung IgE Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
The left lung was collected and homogenized in T-PER tissue protein extraction reagent (Thermo Scientific) supplemented with Halt protease and phosphatase inhibitors (Thermo Scientific) to extract total protein. ELISA MAX Deluxe set mouse total IgE (BioLegend, San Diego CA) was used to quantify IgE levels in both serum and lung tissue homogenate. Lung tissue homogenate immunoglobulin concentrations were normalized to the total protein levels in the sample, quantitated by BCA assay (Pierce, Springfield Township NJ).
Borbet T.C., Pawline M.B., Zhang X., Wipperman M.F., Reuter S., Maher T., Li J., Iizumi T., Gao Z., Daniele M., Taube C., Koralov S.B., Müller A, & Blaser M.J. (2022). Influence of the early-life gut microbiota on the immune responses to an inhaled allergen. Mucosal immunology, 15(5), 1000-1011.
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