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Rna stat reagent

Manufactured by Tel-Test
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RNA STAT reagent is a chemical solution used in molecular biology laboratories to extract and isolate ribonucleic acid (RNA) from various biological samples. The reagent facilitates the lysis of cells and the subsequent purification of RNA, which is essential for various downstream applications such as gene expression analysis, reverse transcription, and RNA sequencing.

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Lab products found in correlation

Iscript cdna synthesis kit, by Bio-Rad (3 mentions) Genechip c elegans genome arrays, by Thermo Fisher Scientific (2 mentions) Nanodrop, by Thermo Fisher Scientific (2 mentions) Revertra ace, by Toyobo (1 mentions) Dnase, by Thermo Fisher Scientific (1 mentions) Myiq2 two color real time pcr detection system, by Bio-Rad (1 mentions) Iq sybr green supermix, by Bio-Rad (1 mentions) Applied biosystems 2720 thermal cycler, by Thermo Fisher Scientific (1 mentions) Superscript vilo kit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

RNA STAT-60 reagent STAT-60 RNA Extraction Reagent RNA-STAT

5 protocols using rna stat reagent

1

Quantitative Real-Time PCR Analysis of Proinflammatory Mediators

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The mRNA expression of proinflammatory mediators was analyzed by quantitative
real-time PCR.23 (link)
 After appropriate stimulation, total RNA was isolated from HMO6 cells
using RNA STAT reagent (Tel-Test, Friendswood, Tx, USA) according to the
manufacturer’s instructions. Two μg of total RNA was reverse transcribed using
reverse transcriptase enzyme (ReverTraAce, Toyobo, Osaka, Japan), and
quantitative real-time PCR was done with SyBr green real-time PCR system
(Applied Biosystem, Warrington WA1 4SR, UK) and gene-specific primers, using an
ABI Prism 7300 Sequence Detection System (Applied Biosystem).
Sheikh A.M., Yano S., Mitaki S., Tabassum S., Yamaguchi S, & Nagai A. (2022). Rho-Kinase inhibition decreases focal cerebral ischemia-induced glial activation in rats. Journal of Central Nervous System Disease, 14, 11795735221123910.
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2

Transcriptional Profiling of atfs-1 Mutant C. elegans

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Wildtype (N2) and atfs-1(et18) worms were synchronized by bleaching, and harvested at the L4 stage of development. Total RNA was extracted using the RNA STAT reagent (Tel-Test Inc.) and used for double-stranded cDNA synthesis using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories). Microarray analysis using GeneChip C. elegans genome arrays (Affymetrix) was conducted as previously described4 (link). Differences in gene expression between wildtype and atfs-1(et18) worms was determined using Anova streamlined (Partek Genomic Suite (v6.5)). Supplementary Table 1 contains the relative fold induction and p values for each mRNA. Confirmation of the microarray results was performed via qRT-PCR as described4 (link). Primers are listed in Supplementary Table 2.
Lin Y.F., Schulz A.M., Pellegrino M.W., Lu Y., Shaham S, & Haynes C.M. (2016). Maintenance and propagation of a deleterious mitochondrial genome by the mitochondrial UPR. Nature, 533(7603), 416-419.
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3

Quantifying Gene Expression by qPCR

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Total RNA was isolated using the RNA STAT reagent (Tel-Test) and treated with DNAse (Ambion). cDNA was then synthesized from total RNA using the iScript™ cDNA Synthesis Kit (Bio-Rad Laboratories). qPCR was used to determine the expression levels of the indicated genes using iQ™ sybr green supermix and MyiQ™2 Two-Color Real-Time PCR Detection System (Bio-Rad). Gene specific primers are listed in Table S6. Actin was used as a control. Fold changes in gene expression were calculated using the comparative CtΔΔCt method.
Nargund A.M., Fiorese C.J., Pellegrino M.W., Deng P, & Haynes C.M. (2015). Mitochondrial and nuclear accumulation of the transcription factor ATFS-1 promotes OXPHOS recovery during the UPRmt. Molecular cell, 58(1), 123-133.
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4

Quantification of Gene Expression from Frozen Liver

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Frozen liver tissue, 5 to 20 mg, was homogenized in 1 ml RNA-STAT reagent (Tel-Test) with isopropanol and EtOH precipitation. RNA pellets were resuspended in 200 μl water and assessed by Nanodrop (ThermoFisher Scientific). RNA, 1 μg, was reverse transcribed into cDNA by Superscript VILO kit (ThermoFisher Scientific), using an Applied Biosystems 2720 Thermal Cycler (ThermoFisher Scientific). qPCR was performed for all samples in duplicate with Power SYBR Green using an Applied Biosystems QuantStudio-3 real-time thermocycler (ThermoFisher Scientific). Target gene Ct values were normalized to reference gene (Rplp0) Ct values by the 2−ΔΔCt method. Oligonucleotide primer sequences are listed in electronic supplementary material Table S1.
Kamm D.R., Pyles K.D., Sharpe M.C., Healy L.N., Colca J.R, & McCommis K.S. (2021). Novel insulin sensitizer MSDC-0602K improves insulinemia and fatty liver disease in mice, alone and in combination with liraglutide. The Journal of Biological Chemistry, 296, 100807.
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5

Transcriptional Profiling of atfs-1 Mutant C. elegans

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Wildtype (N2) and atfs-1(et18) worms were synchronized by bleaching, and harvested at the L4 stage of development. Total RNA was extracted using the RNA STAT reagent (Tel-Test Inc.) and used for double-stranded cDNA synthesis using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories). Microarray analysis using GeneChip C. elegans genome arrays (Affymetrix) was conducted as previously described4 (link). Differences in gene expression between wildtype and atfs-1(et18) worms was determined using Anova streamlined (Partek Genomic Suite (v6.5)). Supplementary Table 1 contains the relative fold induction and p values for each mRNA. Confirmation of the microarray results was performed via qRT-PCR as described4 (link). Primers are listed in Supplementary Table 2.
Lin Y.F., Schulz A.M., Pellegrino M.W., Lu Y., Shaham S, & Haynes C.M. (2016). Maintenance and propagation of a deleterious mitochondrial genome by the mitochondrial UPR. Nature, 533(7603), 416-419.
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