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MHDAC6 is a lab equipment product from Cell Signaling Technology. It is a histone deacetylase 6 (HDAC6) inhibitor.

Automatically generated - may contain errors

Lab products found in correlation

α tubulin, by Merck Group (2 mentions) Pan actin, by Merck Group (2 mentions) Complete protease inhibitor cocktail, by Merck Group (2 mentions) Trans blot turbo transfer system, by Bio-Rad (2 mentions) T9026, by Cell Signaling Technology (2 mentions) Human hdac6, by Cell Signaling Technology (2 mentions) Ag 20b 0042 c100, by Cell Signaling Technology (2 mentions) Amersham ecl western blotting, by Cytiva (2 mentions) Amersham ecl western blotting detection reagent, by Cytiva (1 mentions) Ecl western blotting detection reagent, by Cytiva (1 mentions)

2 protocols using mhdac6

1

Western Blot Analysis of Protein Expression

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Treated BMDM cells were lysed with RIPA buffer (supplied with cOmplete™ Protease Inhibitor Cocktail, Merck#11697498001), and protein concentration was determined by Bradford assay. Same protein amounts were loaded onto 4–12% NuPAGE Gels and protein was transferred to PVDF membranes by using Trans-Blot® Turbo™ Transfer System (Biorad#17001917). Membrane was blocked with 5% milk-TBS, and then incubated with primary antibodies overnight at 4°C. Signal was developed with HRP-conjugated secondary antibodies and Amersham ECL Western Blotting Detection Reagent (Cytiva#RPN2106). Antibody information: mouse Caspase-1 (p20) (AdipoGen#AG-20B-0042-C100), mHDAC6 (homemade), human HDAC6 (CellSignaling#7558), α-tubulin (Sigma-Aldrich #T9026), HA (CellSignaling#3724), pan Actin (Sigma-Aldrich#SAB4502632).
Wang L., Unterreiner A., Kapetanovic R., Aslani S., Xiong Y., Donovan K.A., Farady C.J., Fischer E.S., Bornancin F, & Matthias P. (2023). HDAC6/aggresome processing pathway importance for inflammasome formation is context dependent. bioRxiv.
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2

Western Blot Analysis of Caspase-1 and HDAC6

Check if the same lab product or an alternative is used in the 5 most similar protocols
Treated BMDM cells were lysed with RIPA buffer (supplied with cOmplete Protease Inhibitor Cocktail, Merck#11697498001), and protein concentration was determined by Bradford assay. The same protein amounts were loaded onto 4 to 12% NuPAGE Gels and protein was transferred to PVDF membranes by using Trans-Blot Turbo Transfer System (Biorad#17001917). The membrane was blocked with 5% milk-TBS, and then incubated with primary antibodies overnight at 4 °C. The signal was developed with HRP-conjugated secondary antibodies and Amersham ECL Western Blotting Detection Reagent (Cytiva#RPN2106). Antibody information: mouse Caspase-1 (p20) (AdipoGen#AG-20B-0042-C100), mHDAC6 (homemade), human HDAC6 (CellSignaling#7558), α-tubulin (Sigma-Aldrich #T9026), HA (CellSignaling#3724), pan Actin (Sigma-Aldrich#SAB4502632).
Wang L., Shi S., Unterreiner A., Kapetanovic R., Ghosh S., Sanchez J., Aslani S., Xiong Y., Hsu C.L., Donovan K.A., Farady C.J., Fischer E.S., Bornancin F, & Matthias P. (2024). HDAC6/aggresome processing pathway importance for inflammasome formation is context-dependent. The Journal of Biological Chemistry, 300(2), 105638.
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