Electrode 52 03

Manufactured by Crison

Sourced in Spain

The Crison electrode 52-03 is a pH electrode designed for laboratory use. It is a glass electrode that measures the pH of aqueous solutions. The electrode has a standard BNC connector for easy integration with pH meters and other laboratory equipment.

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Lab products found in correlation

2002 potentiometer, by Crison (4 mentions) 7100 ce instrument, by Agilent Technologies (1 mentions) Chemstation c 01, by Agilent Technologies (1 mentions) J 810 spectropolarimeter, by Julabo (1 mentions) F 25 hd, by Julabo (1 mentions) 8453 spectrophotometer, by Agilent Technologies (1 mentions) Hp 3d ce system, by Agilent Technologies (1 mentions) Ts 100 thermo shaker, by Biosan (1 mentions) Msd ion trap mass spectrometer, by Agilent Technologies (1 mentions)

4 protocols using electrode 52 03

Capillary Electrophoresis with UV Detection

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All CE-UV experiments were
performed on a 7100 CE instrument (Agilent Technologies, Waldbronn,
Germany) with UV absorption diode array detection (DAD). The autosampler
of the CE instrument was kept at the same temperature as the CE cartridge
cassette using an external water bath (Minichiller 300, Peter Huber
Kältemaschinenbau AG, Offenburg, Germany). Data acquisition
was performed with ChemStation C.01.06 software (Agilent Technologies).
CD spectra were recorded on a Jasco J-810 spectropolarimeter equipped
with a Julabo F-25/HD temperature control unit. UV spectra were recorded
on an Agilent 8453 spectrophotometer. Hellma quartz covered cells
(10 mm path length, 3000 mL volume) were used in both cases.
pH measurements were made with a Crison 2002 potentiometer and
a Crison electrode 52–03 (Crison Instruments, Barcelona, Spain).

Bchara L., Eritja R., Gargallo R, & Benavente F. (2023). Rapid and Highly Efficient Separation of i-Motif DNA Species by CE-UV and Multivariate Curve Resolution. Analytical Chemistry, 95(41), 15189-15198.

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CE-UV BGE Preparation with HEC

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The CE‐UV BGE was prepared as described by N. De Jong et al. [1 (link)], substituting methylhydroxyethylcellulose (30 000 M_r) with HEC (250 000 M_r). Sodium citrate dihydrate 10 mM (147 mg) and HEC 0.05% (m/v) (25 mg) were mixed with 37.5 mL of 8 M urea in a 50 mL volumetric flask (i.e. Final urea concentration was 6 M). Then, the pH was adjusted to 2.5 with 2.5 M citric acid solution and the volume was made up with water. The reduction buffer for sample preparation did not contain HEC and was prepared in a similar way, but with DTT (38 mg), less amount of sodium citrate dihydrate (73 mg), and adjusting the pH to 8.0 with sodium hydroxide solution. The CE‐MS BGE contained 2 M HAc (pH 2.2) [18 (link), 19 (link)]. The sheath liquid solution consisted of a hydroorganic mixture of 60:40 (v/v) propan‐2‐ol:water with 0.05% (v/v) of HFor. Both BGEs were passed through 0.20 μm nylon filters (Macherey‐Nagel, Düren, Germany) and the sheath liquid was degassed for 10 min by sonication before the analyses. pH measurements were made with a Crison 2002 potentiometer and a Crison electrode 52‐03 (Crison Instruments, Barcelona, Spain).

Ghafoori Z., Tehrani T., Pont L, & Benavente F. (2022). Separation and characterization of bovine milk proteins by capillary electrophoresis‐mass spectrometry. Journal of Separation Science, 45(18), 3614-3623.

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pH Measurement and Centrifugal Filtration

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The pH measurements were performed with a Crison 2002 potentiometer and a Crison electrode 52-03 from Crison Instruments (Barcelona, Spain). Centrifugal filtration was carried out in a 5417R centrifuge from Eppendorf Ibérica (Madrid, Spain). A Vortex Genius 3 from Ika (Staufen, Germany) was used for agitation.

Pérez-Alcaraz A., Borrull F., Aguilar C., Calull M, & Benavente F. (2021). Enantiodetermination of R,S-3,4-methylenedioxypyrovalerone in urine samples by high pressure in-line solid-phase extraction capillary electrophoresis-mass spectrometry. Talanta, 225.

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CE-MS Analysis of Biomolecule Samples

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All experiments were performed on an Agilent Technologies HP 3D CE system (Waldbronn, Germany) coupled to an MSD Ion Trap mass spectrometer (Agilent Technologies). An electrospray G1603A Agilent Technologies sprayer was used as a sheath-flow-CE-ESI-MS interface. The sheath liquid was delivered by an infusion pump KD Scientific 100 Series (Holliston, MA, USA). The detailed conditions affecting CE-MS have been discussed elsewhere, as has the tuning of the IT mass spectrometer [33] (link). pH measurements were performed with a Crison 2002 potentiometer and a Crison electrode 52-03 (Crison Instruments, Barcelona, Spain). Sample incubation was performed with a Thermo-Shaker TS-100 (Biosan, Warren, USA). Centrifugation was performed in a thermostated Rotanta 460 centrifuge (Hettich Zentrifugen, Tuttlingen, Germany).

Tascon M., Benavente F., Sanz-Nebot V.M, & Gagliardi L.G. (2015). Fast determination of harmala alkaloids in edible algae by capillary electrophoresis mass spectrometry. Analytical and bioanalytical chemistry, 407(13).

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