Axiocam 506 mono ccd camera

Bright and dark field pictures were taken on a Zeiss Axioplan 2 microscope with a SPOT 14.2 camera and SPOT advanced software (Diagnostic Instruments), radioactive in situ hybridization signals were visualized using the illuminator Intralux 5000–1 (Volpi). Fluorescence pictures were taken on a Zeiss Axiovert 200 microscope with a Spot 23.0 camera (Diagnostic Instruments) and Metamorph imaging software (Visitron Imaging Systems) or a Zeiss Axio Observer 7 microscope with an AxioCam 506 mono CCD camera (Zeiss) and Zen 2.3 software (Zeiss).

Erythroid progenitors were seeded at 4–5 × 10⁵ cells/ml on fibronectin‐coated μl‐slide eight‐well ibiTreat (ibidi) and allowed to adhere for 1 h at 37°C, 5% CO₂. Cells were imaged for 48 h at 37°C, 5% CO₂ at a single focal plane in bright field using a Zeiss Axio observer Z1 inverted microscope equipped with an environmental chamber, and either an ORCA‐Flash 4.0 CMOS camera (Hamamatsu) and a Plan‐Apochromat 40×/0.95 Ph3 DIC objective (Zeiss) or an Axiocam 506 mono CCD camera (Zeiss) and a LD Plan‐Neofluar 40×/0.6 Ph2 DIC objective (Zeiss). Zen Blue software (Zeiss) was used for data collection and analysis. To quantify cell cycle timing, the duration between each cytokinesis was scored by following two daughter cells until they stopped dividing, died, or enucleated.

For bright field image acquisition of DAB staining on DRG cryosections, we used a Leica DMI IL LED inverted microscope with a DMC2900 CCD camera (Leica Microsystems, Wetzlar, Germany). Images were acquired with the Leica Application Suite X software (Leica Microsystems, Wetzlar, Germany) at 20× magnification and were used for cell counting of CD11b⁺ and CD3⁺ cells in DRG sections. For fluorescence image acquisition, we used the fluorescence microscope Zeiss Imager M.2 with an Apotome 2 device for structured illumination, Colibri 7 LED as a light source, and an Axiocam 506 mono CCD camera (all Zeiss, Oberkochen, Germany), and the fluorescence microscope Zeiss Axiophot 2 (Zeiss, Oberkochen, Germany) equipped with a SPOT INSIGHT^TM 4.0 Mp Color Camera and the SPOT Advanced Software (both SPOT imaging, Sterling Heights, MI, USA). Images were acquired with the Zeiss ZEN Blue edition software at 20× magnification.