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Viaat cre mice

Manufactured by Jackson ImmunoResearch

Viaat-Cre mice are a genetically modified mouse line that expresses Cre recombinase under the control of the Viaat (Vesicular inhibitory amino acid transporter) promoter. Cre recombinase is an enzyme that can be used to selectively delete or modify target genes in specific cell populations.

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2 protocols using viaat cre mice

1

Genetically Encoded Calcium Indicators in Mice

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VGlut2-Cre mice (Stock No: 016963, Vglut2-cre knock-in; The Jackson Laboratory) were crossed with homozygous floxed GCaMP6s mice (derived from strain Ai96; Stock No: 024106; The Jackson Laboratory, Bar Harbor, ME), resulting in GCaMP6s expression in glutamatergic neurons. To drive expression of GCaMP6s in GABAergic interneurons, we crossed Viaat-Cre mice (Stock No: 016962; The Jackson Laboratory) with homozygous GCaMP6s mice. The mice were kept under standard colony conditions, with 12-h day/night cycles, and had access to food and water ad libitum. Both males and females were used.
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2

Genetically Encoded Calcium Indicators in Mice

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In order to drive GCaMP6s expression in excitatory neurons, VGlut2-Cre mice (Stock No: 028863, Vglut2-cre knock-in, The Jackson Laboratory, Bar Harbor, ME) were crossed with homozygous floxed GCaMP6s mice (derived from strain Ai96, The Jackson Laboratory, Bar Harbor, ME). Expression of GCaMP6s in inhibitory interneurons was achieved by crossing Viaat-Cre mice (Stock No: 017535, The Jackson Laboratory, Bar Harbor, ME) with homozygous GCaMP6s mice. The mice were kept under standard colony conditions, with 12 hour day/night cycles, and had access to food and water ad libitum.
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