Psb36

Manufactured by Bio-Techne

Sourced in United States, United Kingdom

The PSB36 is a power supply unit designed for use in a variety of laboratory applications. It provides a stable and adjustable source of electrical power for powering laboratory equipment such as electrophoresis systems, blotting apparatus, and other devices that require a controlled power supply. The PSB36 features multiple output voltage settings and has over-current protection to ensure safe operation.

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Lab products found in correlation

4 protocols using psb36

Pharmacological Modulation of A1 Receptors

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Experiments were conducted using the A₁-R agonist CCPA (Tocris Bioscience Cat. No. 1705, Abingdon, United Kingdom) dissolved in a mixture of dimethyl sulfoxide (DMSO—Sigma Aldrich Cat. No. PHR1309) and distilled water (ratio 1:100), the A₁-R antagonist PSB36 (Tocris Bioscience Cat. No. 2019, Abingdon, United Kingdom) dissolved in a mixture of DMSO and distilled water (ratio 1:100), the CD73 inhibitor AMPCP (Tocris Bioscience Cat. No. 3633, Abingdon, United Kingdom) dissolved in distilled water. DMSO (1%) was used as vehicle (Figure 1A).

Soattin L., Lubberding A.F., Bentzen B.H., Christ T, & Jespersen T. (2020). Inhibition of Adenosine Pathway Alters Atrial Electrophysiology and Prevents Atrial Fibrillation. Frontiers in Physiology, 11, 493.

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Pharmacological Analysis of Inosine and Adenosine Receptors

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Reagents were purchased from suppliers as indicated: Inosine (Sigma-Aldrich, St. Louis, MO); CGS15943, MRS3777, PSB36, PSB603, ZM241385, PSB0777, NECA, BAY60-6583, repaglinide, iberiotoxin and apamin (Tocris Bioscience, Minneapolis, MN). Inosine was dissolved in distilled water. CGS15943, MRS3777, PSB36, PSB603, ZM241385, PSB0777, NECA, BAY60-6583, iberiotoxin and apamin were dissolved in DMSO and repaglinide was dissolved in ethanol. Exposure of tissues to vehicle alone did not alter spontaneous activity (Supplementary Figure S2). Antagonists were equilibrated in the organ bath for 15 min before the addition of an agonist. The High Capacity Reverse Transcription kit, Universal Master Mix and gene expression assays for rat A_2A (Rn00583935_m1), A_2B (Rn00567697_m1) and GAPDH (Rn01775763_g1) were from Life Technologies (Foster City, CA).

Doyle C., Cristofaro V., Sack B.S., Lukianov S.N., Schäfer M., Chung Y.G., Sullivan M.P, & Adam R.M. (2017). Inosine attenuates spontaneous activity in the rat neurogenic bladder through an A2B pathway. Scientific Reports, 7, 44416.

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Biochemical Reagents Sourcing Protocol

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All chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA), except for ARL 67156, CGS 21680, and PSB-36 (Tocris Bioscience, Ellisville, MO, USA), 1-octanesulfonic acid sodium salt and Mowiol (81381) (Fluka, Steinheim, Germany), and Na₂HPO₄, NaH₂PO₄ and CH₃COONa (POCh, Gliwice, Poland).

Ziemlińska E., Kügler S., Schachner M., Wewiór I., Czarkowska-Bauch J, & Skup M. (2014). Overexpression of BDNF Increases Excitability of the Lumbar Spinal Network and Leads to Robust Early Locomotor Recovery in Completely Spinalized Rats. PLoS ONE, 9(2), e88833.

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Immunomodulatory Agents in T-cell Activation

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Concanavalin A (ConA) were purchased from Sigma–Aldrich. Anti-CD3ε mAb was purchased from R&D Systems (United States). Anti-CD28 mAb was from eBioscience (United States). ATP, ADP, and adenosine were from Sigma (United States). PPADS, BDBD, MRS2578, SCH442416, PSB36, MRS3777, A438079, CGS15943, PSB603, and MRS2111 were from Tocris Bioscience (United Kingdom). Suramin and oxATP were purchased from Sigma–Aldrich. NF449 was from Abcam (United Kingdom). adenosine 5′-[γ-thio]triphosphate tetralithium salt (ATP-γ-S), α,β-methyleneadenosine 5′-triphosphate lithium salt (α,β-Me-ATP), BzATP, α,β-methyleneadenosine 5′-diphosphate sodium salt (α,β-Me-ADP), and 2-methylthioadenosine diphosphate trisodium salt (2-MeS-ADP) were purchased from Sigma–Aldrich. Anti-ERK1/2 mAb and anti-phospho-ERK 1/2 (Thr²⁰²/Tyr²⁰⁴) mAb and other secondary Abs were obtained from Cell Signaling Technology (United States). All other chemicals used were of the highest purity available.

Shinohara Y, & Tsukimoto M. (2018). Adenine Nucleotides Attenuate Murine T Cell Activation Induced by Concanavalin A or T Cell Receptor Stimulation. Frontiers in Pharmacology, 8, 986.

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