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Anti cre

Manufactured by Merck Group
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Anti-Cre is a laboratory reagent used in molecular biology research. It functions as an enzyme inhibitor, specifically targeting the Cre recombinase enzyme. This product is used in various genetic engineering experiments to precisely control and manipulate DNA sequences.

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Lab products found in correlation

Anti neun, by Merck Group (2 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Anti gfap, by Merck Group (1 mentions) Anti ki67, by BD (1 mentions) Anti tubulin β3 tuj1, by BioLegend (1 mentions) Anti β galactosidase, by Promega (1 mentions) Anti pyruvate kinase m2 pkm2, by Cell Signaling Technology (1 mentions) Anti ha, by Santa Cruz Biotechnology (1 mentions) Anti cyclin d1, by Santa Cruz Biotechnology (1 mentions) Anti cd31, by Abcam (1 mentions) Anti ha, by Merck Group (1 mentions) Anti calbindin d 28k, by Merck Group (1 mentions) Anti gfp, by Aves Labs (1 mentions) Anti phospho lkb1, by Cell Signaling Technology (1 mentions) Anti gapdh, by Merck Group (1 mentions) Anti gfp, by Thermo Fisher Scientific (1 mentions) Pcag dsred, by Addgene (1 mentions) Anti h3, by Cell Signaling Technology (1 mentions) Anti h3k27me3, by Merck Group (1 mentions) Anti dsred, by Takara Bio (1 mentions)

Close spelling variants of this product

Mouse anti-Cre (5 citations) Rabbit anti‐Cre (3 citations) Anti-Cre recombinase (2 citations)

7 protocols using anti cre

1

Immunostaining and X-Gal Staining Protocol

Cited 1 time
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Brains were processed by X-Gal staining as described (20 (link)). Immunostaining was carried out as described (21 (link)). Primary antibodies used include: anti-β-galactosidase (Promega), anti-GFP (Aves Lab), anti-Ki67 (BD Biosciences), anti-cyclin D1 and anti-CDKN1B (both from Santa Cruz), anti-HA, anti-Cleaved Caspase-3 and anti-Pyruvate kinase M2 (PKM2) (all from Cell Signaling Technology), anti-CD31 (abcam), anti-Atoh1 and anti-Pax6 (both from DSHB), anti-Tubulin β 3 (TUJ1, BioLegend), anti-cre, anti-NeuN and anti-GFAP (all from EMD Millipore).
Grausam K.B., Dooyema S.D., Bihannic L., Premathilake H., Morrissy A.S., Forget A., Schaefer A.M., Gundelach J.H., Macura S., Maher D.M., Wang K., Heglin A.H., Ge X., Zeng E., Puget S., Chandrasekar I., Surendran K., Bram R.J., Schüller U., Talyor M.D., Ayrault O, & Zhao H. (2017). ATOH1 promotes leptomeningeal dissemination and metastasis of Sonic Hedgehog subgroup medulloblastomas. Cancer research, 77(14), 3766-3777.
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2

Immunohistochemical Profiling of Signaling Proteins

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The following primary antibodies were used for immunohistochemistry: anti-LKB1 (1:100, Merck), anti-phospho-LKB1 (1:50, Cell Signaling Technology), anti-calbindin D-28K (1:2000, Merck), anti-LYK5 (STRAD) (1:600, Abcam), anti-HA (1:1000, Merck), anti-GFP (1:1000, Aves Labs), anti-SIK1 (1:100, LSBio), anti-SIK2 (1:1000, LSBio), anti-SIK3 (1:600, LSBio), anti-Cre (1:300, Merck), anti-Robo2 (1:100, Tamada et al., 2008) and anti-pan-gamma-protocadherin (1:200, NeuroMab). All secondary antibodies listed in the KEY RESOURCES TABLE were used at a 1:1000 dilution.
Kuwako K.I, & Okano H. (2018). The LKB1-SIK Pathway Controls Dendrite Self-Avoidance in Purkinje Cells. Cell reports, 24(11).
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3

Molecular Markers for Neuronal Lineages

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Plasmid pCAG-dsRed was from Addgene (Plasmid #11151). Neurod1-Cre was provided by Dr. Franck Polleux (Columbia University). Pak3K297R was gifted by Dr. Rick Horwitz (University of Virginia). The PAK3 mutant was subcloned into the pCMVmyc vector.
The following primary antibodies were used: anti-EZH2 1:500 (BD Biosciences, San Jose, USA, Cat. No. 612666), anti-EZH2 1:1000 (Cell Signaling, Danvers, USA, Cat. No. #5246), anti-GAPDH 1:5000 (Sigma-Aldrich, St. Louis, USA, Cat. No. G8795), anti-GFP 1:1200 (Thermo Fisher Scientific, Waltham, USA, Cat. No. A10262), anti-TUJ1 1:1000 (Biolegend, San Diego, USA, Cat. No. 801201), anti-H3K27me3 1:2000 (Millipore, Burlington, USA, Cat. No. 07449), anti-H3 1:1000 (Cell Signaling, Cat. No. #4499), anti-dsRED 1:1000 (Clontech, Mountain View, USA, Cat. No. 632496), anti-Tbr1 1:1000 (Abcam, Waltham, USA, Cat. No. ab31940), anti-Ctip2 1:500 (Abcam, Cat. No. ab18465), anti-Foxp1 1:1000 (Abcam, Cat. No. ab16645), and anti-Cre 1:1000 (Millipore, Cat. No. MAB3120).
Zhang M., Zhang Y., Xu Q., Crawford J., Qian C., Wang G.H., Qian J., Dong X.Z., Pletnikov M.V., Liu C.M, & Zhou F.Q. (2023). Neuronal Histone Methyltransferase EZH2 Regulates Neuronal Morphogenesis, Synaptic Plasticity, and Cognitive Behavior in Mice. Neuroscience Bulletin, 39(10), 1512-1532.
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4

Immunostaining Markers in Auditory Research

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The primary antibodies used in this study were: anti-GFP (1:1000, chicken, Abcam), anti-Myosin 7a (1:200, rabbit, Proteus), anti-Sox10 (1:250, goat, Santa Cruz Biotechnology), anti-Jag1 (1:75, rabbit, Santa Cruz Biotechnology), anti-NeuN (1:500, mouse, Millipore), anti-GFAP (1:500, rabbit, Dako Z0334) and anti-Cre (1:150, mouse, Millipore). The secondary antibodies used in this study include Alexa Fluor 488 or Alexa Fluor 596 conjugated goat anti rabbit IgG, goat anti mouse IgG, goat anti chicken IgG or donkey anti goat IgG, respectively (1:200, Invitrogen). The immunohistochemistry procedure followed standard protocols. The sections and isolated cochlear whole mount samples were blocked with PBS containing 10% normal goat serum or 5% normal donkey serum and 0.2% Triton X-100 for one hour at room temperature, then incubated with primary antibody overnight at 4°C. After rinsing with PBS, the tissues were incubated with secondary antibody for one hour at room temperature, followed by DAPI staining (1:1000 in PBS) for 15 minutes, then mounted with Fluoromount. For 3D reconstruction of Sox2 expression in the otocyst, sections stained with Sox2 antibodies were processed for horseradish peroxidase-based visualization using a Vectastain kit and DAB.
Gu R., Brown RM I.I., Hsu C.W., Cai T., Crowder A., Piazza V.G., Vadakkan T.J., Dickinson M.E, & Groves A.K. (2016). Lineage tracing of Sox2-expressing progenitor cells in the mouse inner ear reveals a broad contribution to non-sensory tissues and insights into the origin of the organ of Corti. Developmental biology, 414(1), 72-84.
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5

Analyzing Retinal Protein Levels

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Retinal homogenates from the adult mutant mice were analyzed by Western blotting. The primary antibodies used for Western blotting were anti-ELKS (1:500, rabbit, [41 (link)]), anti-CAST (1:1000, rabbit, [42 (link)]), anti-Cre (1:500, rabbit, 69050-3, Millipore), and anti-GAPDH (1:5000, HRP conjugate, #3683, Cell Signaling Technology, Danvers, MA, USA).
Hagiwara A., Mizutani A., Kawamura S., Abe M., Hida Y., Sakimura K, & Ohtsuka T. (2023). Critical Role of the Presynaptic Protein CAST in Maintaining the Photoreceptor Ribbon Synapse Triad. International Journal of Molecular Sciences, 24(8), 7251.
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6

Immunofluorescent Labeling of Cellular Proteins

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Cells were fixed for 15 min at room temperature in 4% (w/v) paraformaldehyde in PBS, washed 3 times in PBS, then permeabilized for 1 hr in Permeabilization buffer (PB: 0.3% Triton X-100, 0.3% BSA (Sigma), in PBS). Primary antibodies were incubated for 2 hr at room temperature in PB. Secondary antibodies were incubated for 1 hr at room temperature. Coverslips (BioCoat) were mounted on slides with Fluoromount G (EMS). Primary antibodies used for immunohistochemistry and immunocytochemistry in this study are chicken anti-GFP (Rockland) (1:2000) and anti-CRE (Millipore) (1:1000). All secondary antibodies were Alexa-conjugated (Invitrogen) and used at a 1:1000 dilution. Nuclear DNA was stained using Hoechst 33258 (1:10,000, Pierce).
Lanfranchi M., Meyer-Dilhet G., Reis R.D., Garcia A., Blondet C., Javin L., Amar A., & Courchet J. (2020). The AMPK-related kinase NUAK1 controls cortical axons branching though a local modulation of mitochondrial metabolic functions.
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7

Embryonic Cardiac Development Analysis

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E15.5 and E18.5 embryos were examined under the stereomicroscope, fixed overnight in 4% paraformaldehyde (PFA) and E15.5 embryos embedded in paraffin, sectioned, and stained with eosin and hematoxylin. E18.5 hearts and great vessels were manually dissected and photographed under a stereomicroscope, and then embedded in paraffin, sectioned, and stained. Immunofluorescence Embryos were fixed overnight in 4% PFA and embedded in wax. For immunofluorescence analysis embedded embryos were cutted in 7µm sections. Sections were deparaffinized in xilene, rehydrated, and after antigen unmasking with citrate buffer, sections were incubated overnight at room temperature with primary antibodies (in 0.5% milk, 10% fetal bovine serum, 1% bovine serum albumin in H2O). Each experiment was repeated at least three times.
We used the following primary antibodies: Anti-GFP (Abcam ab13970, diluted 1:1000), Anti-SLUG (Cell Signaling, #9585, diluted 1:100); Anti-TFAP2A (Hybridoma Bank, clone 3B5, diluted 1:300); Anti-ISL1, (Hybridoma Bank, clone 39.4D5, diluted 1:100); Anti-CRE (Millipore, 69050-3, diluted 1:1000); Anti-TBX1 (Abcam, Ab18530, diluted 1:100).
Lania G., Franzese M., Adachi N., Bilio M., Russo A., d'Agostino E., Angelini C., Kelly R.G., & Baldini A. (2021). A phenotypic rescue approach identifies lineage regionalization defects in a mouse model of DiGeorge syndrome.
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