E64d 4321 v

Manufactured by Peptide Institute

E64d (4321-v) is a laboratory reagent used as a protease inhibitor. It functions by inhibiting the activity of cysteine proteases. This product is commonly utilized in research applications to study the role of cysteine proteases in various biological processes.

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Lab products found in correlation

Pepstatin a 4397 v, by Peptide Institute (2 mentions) Fugene hd transfection reagent, by Promega (1 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Fujifilm (1 mentions) Rapamycin tlrl rap, by InvivoGen (1 mentions) Chloroquine diphosphate salt c6628, by Merck Group (1 mentions) Avagacestat, by Selleck Chemicals (1 mentions) Ly411575, by Merck Group (1 mentions) Bafilomycin a1 b1793, by Merck Group (1 mentions) Anti ha rat monoclonal antibody, by Roche (1 mentions) Mouse monoclonal anti actin antibody, by Merck Group (1 mentions) Ro4929097, by Selleck Chemicals (1 mentions) Mk 0752, by Selleck Chemicals (1 mentions) Dapt gsi 9, by Selleck Chemicals (1 mentions) Semagacestat, by Selleck Chemicals (1 mentions)

2 protocols using e64d 4321 v

Huh7.5.1 Cell Culture and Antibodies for Autophagy

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KOD-plus- (KOD-201) was employed for high-fidelity polymerase chain reactions (TOYOBO). Huh7.5.1 cells derived from the Huh7 cell line (ATCC CCL-185) were cultured in a Dulbecco's modified Eagle Medium (DMEM; Wako, 045-30285) containing 10% fetal calf serum (JRH biosciences/SIGMA, 12603C) and 1% nonessential amino acids (Invitrogen, 11140050). Polyclonal antibodies against human LC3 was described previously [10] (link). The mouse monoclonal antibody, clone M2, against FLAG peptide (DYKDDDDK) (F1804), ammonium chloride (254134), and chloroquine diphosphate salt (C6628) were purchased from SIGMA-ALDRICH, the mouse monoclonal antibodies against GAPDH (ab8245) were from Abcam, and the rabbit monoclonal antibody against Myc epitope tag (2278) was from Cell Signaling Technology. Protein concentrations were determined using the bicinchoninic acid protein assay reagent (Pierce, 23225). E64d (4321-v) and pepstatin A (4397-v) were purchased from Peptide Institute, and the rapamycin (#tlrl-rap) was from InvivoGen. Torin1 was a kind gift from Dr. Nathanael S. Gray at the Dana Farber Cancer Institute and Dr. David Sabatini at the Whitehead Institute for Biomedical Research. To introduce the plasmid into the cells, FuGENE HD transfection reagent (E2311) was used (Promega).

Tanida I., Ueno T, & Uchiyama Y. (2014). A Super-Ecliptic, pHluorin-mKate2, Tandem Fluorescent Protein-Tagged Human LC3 for the Monitoring of Mammalian Autophagy. PLoS ONE, 9(10), e110600.

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Antibody Sources for HCV Research

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The following antibodies were obtained: anti-HCV NS5A mouse monoclonal antibody (5A27)15 (link), anti-HCV core mouse monoclonal antibody (Fujirebio, Japan), anti-actin mouse monoclonal antibody (Sigma, A2228), anti-HA rat monoclonal antibody (Roche, clone 3F10), anti-GFP mouse monoclonal antibody (Clontech, JL-8), anti-E1 mouse monoclonal antibody (toru0726cb-1)51 (link), anti-E6AP antibody (Sigma), anti-PA28γ antibody52 (link), anti-p62 antibody (MBL) and horseradish peroxidase-conjugated anti-FLAG mouse monoclonal antibody (Sigma, clone M2). Anti-SPP rabbit polyclonal antibody was generated by immunization with synthetic peptides from amino acids 356 to 366 of mouse SPP (YEESNPKDPAA). LY-411575, ALLN (A6185), MG132, and bafilomycin A1 (B1793) were obtained from Sigma. RO4929097, avagacestat, semagacestat, DAPT (GSI-IX), and MK-0752 were purchased from Selleck Chemicals. Epoxomicin (4381-v), lactacystin (4368-v), E-64d (4321-v), and pepstatin A (4397-v) were from Peptide Institute, Inc.

Aizawa S., Okamoto T., Sugiyama Y., Kouwaki T., Ito A., Suzuki T., Ono C., Fukuhara T., Yamamoto M., Okochi M., Hiraga N., Imamura M., Chayama K., Suzuki R., Shoji I., Moriishi K., Moriya K., Koike K, & Matsuura Y. (2016). TRC8-dependent degradation of hepatitis C virus immature core protein regulates viral propagation and pathogenesis. Nature Communications, 7, 11379.

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