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Spe supelcleantm columns

Manufactured by Merck Group
Sourced in United States

SPE SupelcleanTM columns are solid-phase extraction (SPE) columns designed for sample preparation. They provide a simple and efficient method for the extraction, purification, and concentration of analytes from complex matrices. The columns contain a sorbent material that selectively retains target compounds, allowing for their separation from interfering substances. The core function of these columns is to facilitate the sample preparation process prior to analytical testing, but a detailed description of their intended use is not provided to maintain an unbiased and factual approach.

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3 protocols using spe supelcleantm columns

1

Antifungal Susceptibility Assay Protocol

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Roswell Park Memorial Institute (RPMI) 1640 medium (Sigma-Aldrich, St. Louis, MA, USA) (with MOPS, pH 7.2), dimethylsulfoxide (DMSO), Sabouraud dextrose agar (SDA; Difco), saline solution (SS), iMarKTM microplate reader (Bio-Rad, Hercules, CA, USA), FLC, Bioscreen C equipment, Bioscreen software (Growth Curves USA, Piscataway, NJ, USA), 100-well microplates (honeycomb), LIVE/DEAD™ Yeast Viability kit (Thermo Fisher Scientific, Waltham, MA, USA), Olympus FV 1000 confocal microscope, Tescan Lyra 3 microscope, Fmoc-Arg(Pbf)–OH, Fmoc-Trp(Boc)–OH, Fmoc-Gln(Trt)–OH, Fmoc-Leu-OH, Fmoc-Lys(Fmoc)–OH, Fmoc-6-Ahx-OH, Rink amide resin, dicyclohexilcarbodiimide (DCC), and 1-hydroxy-6-chlorobenzotriazole were purchased from AAPPTec (Louisville, KY, USA). Trifluoroacetic acid (TFA), acetonitrile (ACN), dichloromethane (DCM), N,N-dimethylformamide, ethanodithiol, triisopropylsilane, methanol, acetonitrile, and isopropanol were obtained from Merck (Darmstadt, Germany). SPE SupelcleanTM columns were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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2

Antifungal Susceptibility Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Roswell Park Memorial Institute (RPMI) 1640 medium (Sigma-Aldrich, St. Louis, MA, USA) (with MOPS, pH 7.2), dimethylsulfoxide (DMSO), Sabouraud dextrose agar (SDA; Difco), saline solution (SS), iMarKTM microplate reader (Bio-Rad, Hercules, CA, USA), FLC, Bioscreen C equipment, Bioscreen software (Growth Curves USA, Piscataway, NJ, USA), 100-well microplates (honeycomb), LIVE/DEAD™ Yeast Viability kit (Thermo Fisher Scientific, Waltham, MA, USA), Olympus FV 1000 confocal microscope, Tescan Lyra 3 microscope, Fmoc-Arg(Pbf)–OH, Fmoc-Trp(Boc)–OH, Fmoc-Gln(Trt)–OH, Fmoc-Leu-OH, Fmoc-Lys(Fmoc)–OH, Fmoc-6-Ahx-OH, Rink amide resin, dicyclohexilcarbodiimide (DCC), and 1-hydroxy-6-chlorobenzotriazole were purchased from AAPPTec (Louisville, KY, USA). Trifluoroacetic acid (TFA), acetonitrile (ACN), dichloromethane (DCM), N,N-dimethylformamide, ethanodithiol, triisopropylsilane, methanol, acetonitrile, and isopropanol were obtained from Merck (Darmstadt, Germany). SPE SupelcleanTM columns were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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3

Peptide Synthesis and Cell Viability

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The HTB-132 MCF-7 and MCF-12 cell lines were obtained from ATCC® (Manassas, VA, USA); LIVE/DEADTM BacLightTM Viability Kit, and annexin V, Alexa FluorTM 488 conjugate was purchased from Invitrogen (Eugene, Oregon); Mitochondrial Membrane Potential Detection was obtained from BD Biosciences (Torreyana Rd., San Diego, CA 92121, USA). The DMEM culture medium was obtained from Sigma-Aldrich (St. Louis, MO, USA). Bovine fetal serum was purchased in Gibco (Waltham, MA, USA). The Fmoc-amino acids, Rink amide resin, dicyclohexylcarbodiimide and 1-hydroxy-6-chlorobenzotriazole were purchased from AAPPTec (Louisville, KY, USA). Acetonitrile, trifluoroacetic acid, dichloromethane, diisopropylethylamine, N, N-dimethylformamide, ethanedithiol, isopropanol, methanol, triisopropylsilane were purchased from Merck (Darmstadt, Germany). SPE SupelcleanTM columns were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Silicycle® SiliaPrepTM C18 columns were kindly donated by EcoChem Especialidades Químicas (Waterloo, QC, Canada).
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