Log In Sign up
The largest database of trusted experimental protocols

Anti cyp8b1

Manufactured by Santa Cruz Biotechnology
Visit Supplier

Anti-Cyp8b1 is a laboratory reagent used for the detection and quantification of the Cyp8b1 protein. Cyp8b1 is a cytochrome P450 enzyme involved in bile acid synthesis. The Anti-Cyp8b1 product can be used in various applications such as Western blotting, immunohistochemistry, and ELISA to analyze the expression and localization of the Cyp8b1 protein in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

S 100, by Santa Cruz Biotechnology (2 mentions) Enhanced chemiluminescence kit, by Thermo Fisher Scientific (1 mentions) Anti β actin, by Bioworld Technology (1 mentions) Anti pkac, by Santa Cruz Biotechnology (1 mentions) Anti p ser, by Santa Cruz Biotechnology (1 mentions) Anti cyp7a1, by Santa Cruz Biotechnology (1 mentions) Anti pkar, by Santa Cruz Biotechnology (1 mentions) Anti hnf4α, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

CYP8B1 (3 citations)

3 protocols using anti cyp8b1

1

Immunohistochemical Detection of Cyp8b1

Check if the same lab product or an alternative is used in the 5 most similar protocols
Formalin-fixed and paraffin-embedded livers were sectioned (5μm), deparaffinized, rehydrated and permeabilized in 0.3% hydrogen peroxide in PBS for 20min at RT. Antigen retrieval was carried out by boiling in citrate buffer (pH=6) for 10min in a pressure cooker. Sections were blocked with normal goat serum (normal goat serum Vector labs, S-100) and incubated for 1h at RT with anti-Cyp8b1 (Santa Cruz, sc-23515, diluted 1:50 in PBS). Detection was performed by incubation with a peroxidase-conjugated anti-rabbit antibody (Zytomed Systems, ZUC032) using DAB as chromogen.
Halpern K.B., Shenhav R., Matcovitch-Natan O., Toth B., Lemze D., Golan M., Massasa E.E., Baydatch S., Landen S., Moor A.E., Brandis A., Giladi A., Avihail A.S., David E., Amit I, & Itzkovitz S. (2017). Single-cell spatial reconstruction reveals global division of labor in the mammalian liver. Nature, 542(7641), 352-356.
+ Open protocol
+ Expand
2

Immunohistochemical Detection of Cyp8b1

Check if the same lab product or an alternative is used in the 5 most similar protocols
Formalin-fixed and paraffin-embedded livers were sectioned (5μm), deparaffinized, rehydrated and permeabilized in 0.3% hydrogen peroxide in PBS for 20min at RT. Antigen retrieval was carried out by boiling in citrate buffer (pH=6) for 10min in a pressure cooker. Sections were blocked with normal goat serum (normal goat serum Vector labs, S-100) and incubated for 1h at RT with anti-Cyp8b1 (Santa Cruz, sc-23515, diluted 1:50 in PBS). Detection was performed by incubation with a peroxidase-conjugated anti-rabbit antibody (Zytomed Systems, ZUC032) using DAB as chromogen.
Halpern K.B., Shenhav R., Matcovitch-Natan O., Toth B., Lemze D., Golan M., Massasa E.E., Baydatch S., Landen S., Moor A.E., Brandis A., Giladi A., Avihail A.S., David E., Amit I, & Itzkovitz S. (2017). Single-cell spatial reconstruction reveals global division of labor in the mammalian liver. Nature, 542(7641), 352-356.
+ Open protocol
+ Expand
3

Western Blot Analysis of Liver Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins were extracted following the procedure described previously (22 (link)). The proteins were separated by SDS-PAGE on 8–12% polyacrylamide gels and subsequently electrically transferred to a PVDF membrane. After blocking with 5% (w/v) BSA in TBST at room temperature for 1 h, the membranes were then incubated with an appropriate specific primary antibody (anti-CYP7A1, Santa Cruz, Cat# sc-518007, 1:500; anti-PER1, Sigma-Aldrich, Cat# AB2201, 1:200; anti-CYP8B1, Santa Cruz, Cat# sc-101387,1:500; anti-CYP27A1, Santa Cruz, Cat# sc-390974,1:500; anti-HNF-4α, Santa Cruz, Cat# sc-101059,1:500; anti-PKA-C, Santa Cruz, Cat# sc-365615,1:500; anti-PKA-R, Santa Cruz, Cat# sc-271125,1:500; anti-β-actin, BioWorld, Cat# AP0060, 1:1000; anti-p-Ser, Santa Cruz, Cat# sc-81514) at 4°C overnight, followed by incubation with an HRP-conjugated secondary antibody (1:4000). Detection was performed using an enhanced chemiluminescence kit (Thermo Scientific, Hudson, NH).
Ge W., Sun Q., Yang Y., Ding Z., Liu J, & Zhang J. (2023). Circadian PER1 controls daily fat absorption with the regulation of PER1-PKA on phosphorylation of bile acid synthetase. Journal of Lipid Research, 64(6), 100390.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!