A 10-mL peripheral blood sample was drawn from each subject, after an overnight fasting for 12 h. The Biosystem A15 auto-analyzer with different enzymatic colorimetric methods was used to assess concentrations of some special biochemical parameters including: (1) triglyceride (TG) (glycerol phosphate oxidase (GPO)); (2) fasting blood glucose (FBG) (glucose oxidase (GOD)); (3) high-density lipoprotein cholesterol (HDL-c) (cholesterol oxidase (CHOD)). Other serum parameters were also measured using the commercial enzyme-linked immunosorbent assay (ELISA) kits as follows: (1) high sensitive C-reactive protein (hs-CRP) (turbidimetry kit, latex enhanced turbidimetric method, Delta.DP); (2) insulin (Monobined Inc. Lake Forest, CA 92630, USA); (3) BDNF and adropin (Zellbio, Veltlinerweg, Germany). Insulin resistance (IR) was calculated through Homeostasis Model Assessment Insulin Resistance (HOMA-IR) formula: HOMA-IR = [FBG (mmol/L) × fasting insulin (mU/L)]/22.537 (link). First decile of serum BDNF concentration was considered as low serum BDNF levels (serum BDNF < 0.47 ng/mL).
Brain derived neurotrophic factor (bdnf)
Manufactured by ZellBio
Sourced in United States, Germany
BDNF is a laboratory-grade reagent used for research purposes. It is a protein that plays a key role in the growth, development, and maintenance of nerve cells.
Automatically generated - may contain errors
4 protocols using brain derived neurotrophic factor (bdnf)
1
Assessing Biochemical Parameters in Fasting Subjects
2
Serum Biomarker Assessment in Fasting Participants
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After 12 h of an overnight fasting, a 10-ml blood sample was collected from each participant. Blood samples were immediately centrifuged at 3500 rpm for 10 min to separate serum. These serum samples were kept at -80° C for further tests. The serum levels of FBG, TG, and HDL-cholesterol were assessed using Biosystem A15 autoanalyzer with different colorimetric methods. The commercial enzyme-linked immunosorbent assay (ELISA) kits were used for assessment of hs-CRP (turbidimetry kit, latex enhanced turbidimetric method, Delta.DP), insulin (Monobined Inc. Lake Forest, CA 92630, USA), BDNF (Zellbio, Veltlinerweg, Germany) and adropin (Zellbio, Veltlinerweg, Germany). HOMA-IR equation described by Matthews et al.22 (link) was used to determine insulin resistance (IR): HOMA-IR = [FBG (mmol/L) × fasting insulin (mU/L)]/22.5.
3
Biomarker Quantification in Brain Tissue
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Furthermore, brain tissue samples were provided and located in a cold buffer (0.1 M phosphate-buffered saline, pH 7.4, involving protease inhibitor cocktail (Roche)); for 15 minutes, it was homogenized and centrifuged at 3500 rpm, and supernatants were gathered. TNF-α (Zellbio, Germany), C-reactive protein (CRP) (Zellbio, Germany), BDNF (Zellbio, Germany), total antioxidant capacity (TAC) (Zellbio, Germany), and total oxidant capacity (TOC) (Zellbio, Germany) were measured utilizing special kits.
4
Comprehensive Biochemical Profiling Protocol
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Following a 12-hour overnight fasting, blood samples will be collected from all participants. A 10 mL sample of peripheral blood will be collected via venipuncture and then will be centrifuged at 3500 rpm within 10 min to separate the serum. Assessment of biochemical parameters will be carried out by the use of special enzymatic colorimetric methods and Biosystem A15 autoanalyser, as follows: (1) concentration of fasting blood glucose (FBG) using glucose oxidase; (2) serum levels of total cholesterol, HDL cholesterol and low-density lipoprotein cholesterol using cholesterol oxidase; (3) serum levels of triglyceride (TG) using glycerol phosphate oxidase; (4) serum levels of uric acid using uricase-peroxidase. The commercial ELISA kits will also be used to measure the concentrations of some parameters including the following: (1) BDNF and adropin (Zellbio, Veltlinerweg, Germany); (2) 25-hydroxy vitamin D and insulin (Monobined, Lake Forest, California, USA); (3) malondialdehyde, superoxide dismutase and glutathione peroxidase (Kiazist Co, Hamedan, Iran); (4) creatinine (Pars Azmoon, Tehran, Iran); (5) C reactive protein (turbidimetry kit, latex enhanced turbidimetric method, Delta.DP).
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