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2 protocols using histidine biotin

1

Mutagenicity Evaluation Assay Protocol

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Dimethyl sulfoxide (DMSO), 2-aminoanthracen (2AA), 4-nitroquinoline-oxide (4NQO), sodium azide (SA), agar, glucose, histidine/biotin, oxoid nutrient broth no.2, and NaCl were purchased from Sigma-Aldrich Company (Bellefonte, PA, USA). The S9 fraction of rat liver and co-factors for the NADH regenerating system were purchased from Mol-Tox (Boone, NC, USA) and Wako (Osaka, Japan). All other chemicals and the used reagents were of high-performance liquid chromatography.
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2

Comprehensive Reagent Inventory for Bioassays

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Laboratory reagents including ethanol, water, Folin-Ciocalteu’s phenol reagent. gallic acid, quercetin, phosphate-buffered saline (PBS), neutral red, dimethyl sulfoxide (DMSO), benzo[a]pyrene (BaP), 4-nitroquinoline n-oxide (4nqo), sodium azide (SA), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonate (ABTS), agar, glucose, histidine/biotin, nutrient broth no. 2, NaCl, Lipopolysaccharide (LPS), and phorbol 12-myristate 13-acetate (PMA) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). The co-factors for the NADH regenerating system and the S9 fraction of rat liver was purchased from Wako (Osaka, Japan) and Mol-Tox (Annapolis, MD, USA). EZ-CYTOX kit for cytotoxicity test is available from DoGenBio Co. (Seoul, Korea), and the ELISA kit for inflammation test was purchased from Abcam (Cambridge, UK). All other chemicals and reagents used were of analytical grade.
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