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Zo 1 rabbit polyclonal antibody

Manufactured by Abcam
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ZO-1 rabbit polyclonal antibody is a laboratory reagent used to detect the ZO-1 protein. ZO-1 is a tight junction-associated protein that plays a role in the regulation of epithelial and endothelial cell permeability.

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Lab products found in correlation

E cadherin rabbit monoclonal antibody, by Cell Signaling Technology (1 mentions) Image pro plus 6, by Media Cybernetics (1 mentions)

Spelling variants of this product

ZO-1 antibody (8 citations) Rabbit anti-ZO-1 polyclonal antibody (2 citations)

2 protocols using zo 1 rabbit polyclonal antibody

1

Immunohistochemical Profiling of Tissue Markers

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Formalin-fixed tissue was embedded in paraffin and stained with F4/80 rat monoclonal antibody (Novus, NB600-404), CD11b rabbit polyclonal antibody (Novus Cat# NB110-89474), Ly-6G/Ly-6C rat monoclonal antibody (Novus Cat# NBP2-00441), E-cadherin rabbit monoclonal antibody (Cell Signaling Technology Cat# 3195), ZO-1 rabbit polyclonal antibody (Abcam Cat# ab59720), Occludin rabbit polyclonal antibody (Novus Cat# NBP1-87402) and Claudin-1 rabbit polyclonal antibody (Abcam Cat# ab15098).
Zou D., Pei J., Lan J., Sang H., Chen H., Yuan H., Wu D., Zhang Y., Wang Y., Wang D., Zou Y., Chen D., Ren J., Gao X, & Lin Z. (2020). A SNP of bacterial blc disturbs gut lysophospholipid homeostasis and induces inflammation through epithelial barrier disruption. EBioMedicine, 52, 102652.
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2

Immunofluorescent Staining of Tight Junctions

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After deparaffinization of jejunal tissue sections fixed with 4% paraformaldehyde, ethylenediaminetetraacetic acid (EDTA, 1 mol/L, pH 9.0, Gooddbio Technology CO., LTD., Wuhan, China) was added to extract antigens. Tissues were permeabilized with 0.5% Triton X-100, and blocked with 3% bovine serum albumin (BSA), before being incubated with the ZO-1 rabbit polyclonal antibody (1:200; Abcam Plc., Cambridge, UK), goat anti-rabbit IgG-FITC (Gooddbio Technology Co., Ltd., Wuhan, China). Next, the nucleus was stained with 4’-6-diamidino-2-phenylindole (DAPI, Gooddbio Technology Co., Ltd., Wuhan, China) in the dark, and the excess DAPI were washed away for confocal laser scanning. Microscopic observation was followed by statistical analysis image-pro plus 6.0 (Media Cybernetics, Inc., Rockville, MD, USA).
Zhou Y., Luo Y., Yu B., Zheng P., Yu J., Huang Z., Mao X., Luo J., Yan H, & He J. (2022). Agrobacterium sp. ZX09 β-Glucan Attenuates Enterotoxigenic Escherichia coli-Induced Disruption of Intestinal Epithelium in Weaned Pigs. International Journal of Molecular Sciences, 23(18), 10290.
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