Hrp goat anti mouse anti rabbit igg

Cell lysate was prepared using RIPA lysis buffer (Applygen, Beijing, China) and proteins were extracted. SDS-PAGE (10%) was used to separate the extracted proteins. After that, proteins were transferred onto PVDF membranes. Nonspeci c antigen was blocked by 5% non-fat milk solution, and the membranes were washed three times using PBST. The membranes were then incubated overnight at 4°C with anti-NDRG2 (12015-1-AP, Proteintech, Rosemont, IL, USA) and anti-β-actin (60008-1-Ig, Proteintech). After washing with PBST, the membranes were incubated with appropriate HRP goat anti-mouse/anti-rabbit IgG (Proteintech) at room temperature. The visualization of all the blots were conducted using enhanced chemiluminescence (ECL; Thermo Fisher).

Cell lysate was prepared using RIPA lysis buffer (Applygen, Beijing, China) and proteins were extracted. SDS-PAGE (10%) was used to separate the extracted proteins. After that, proteins were transferred onto PVDF membranes. Nonspeci c antigen was blocked by 5% non-fat milk solution, and the membranes were washed three times using PBST. The membranes were then incubated overnight at 4°C with anti-NDRG2 (Cat# 12015-1-AP, Proteintech, Rosemont, IL, USA), anti-BAX antibody (1:1000, Cat# ab32503), anti-BCL2 antibody (1:1000, Cat# ab32124), anti-Cleaved Caspase-3 antibody (Cat# ab2302), anti-Caspase-3 antibody (1:500, Cat# ab13847), anti-cyclin B1 (1:50000, Cat# ab32053), anti-cyclin A2 (1:2000, Cat# ab181591) (Abcam, Cambridge, MA, USA) and anti-β-actin (60008-1-Ig, Proteintech). After washing with PBST, the membranes were incubated with appropriate HRP goat anti-mouse/anti-rabbit IgG (Proteintech) at room temperature. The visualization of all the blots were conducted using enhanced chemiluminescence (ECL; Thermo Fisher).