Participants were instructed to visit the hospital after 12 hours of fasting.
All visits occurred in the morning, before any vigorous physical activity.
Blood samples were collected to obtain a hemogram and levels of glucose,
insulin, total cholesterol (TC), high-density lipoprotein cholesterol
(HDL-c), low-density lipoprotein cholesterol (LDL-c), triacylglycerol (TAG),
human adiponectin, and C-reactive protein (CRP). Data were collected by
venipuncture and the blood samples were stored in dry tubes for biochemical
measurements. The colorimetric enzymatic method was used to measure TC,
HDL-c, and TAG. The cutoff points were those proposed to Brazilian children
and adolescents21 (link). LDL-c
was calculated using the Friedewald et al.22 (link) formula. The ultra-sensitive CRP was
measured by the turbidimetric assay and adiponectin, by the ELISA method
(Adiponectin DuoSet, R&D Systems, Inc., Minneapolis, MN, USA).
All visits occurred in the morning, before any vigorous physical activity.
Blood samples were collected to obtain a hemogram and levels of glucose,
insulin, total cholesterol (TC), high-density lipoprotein cholesterol
(HDL-c), low-density lipoprotein cholesterol (LDL-c), triacylglycerol (TAG),
human adiponectin, and C-reactive protein (CRP). Data were collected by
venipuncture and the blood samples were stored in dry tubes for biochemical
measurements. The colorimetric enzymatic method was used to measure TC,
HDL-c, and TAG. The cutoff points were those proposed to Brazilian children
and adolescents21 (link). LDL-c
was calculated using the Friedewald et al.22 (link) formula. The ultra-sensitive CRP was
measured by the turbidimetric assay and adiponectin, by the ELISA method
(Adiponectin DuoSet, R&D Systems, Inc., Minneapolis, MN, USA).