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Adiponectin duoset

Manufactured by R&D Systems
Sourced in United States

The Adiponectin DuoSet is a sandwich enzyme-linked immunosorbent assay (ELISA) kit designed for the quantitative measurement of Adiponectin. Adiponectin is a protein hormone secreted by adipose tissue that plays a role in regulating glucose and lipid metabolism.

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2 protocols using adiponectin duoset

1

Metabolic Profile of Fasted Participants

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Participants were instructed to visit the hospital after 12 hours of fasting.
All visits occurred in the morning, before any vigorous physical activity.
Blood samples were collected to obtain a hemogram and levels of glucose,
insulin, total cholesterol (TC), high-density lipoprotein cholesterol
(HDL-c), low-density lipoprotein cholesterol (LDL-c), triacylglycerol (TAG),
human adiponectin, and C-reactive protein (CRP). Data were collected by
venipuncture and the blood samples were stored in dry tubes for biochemical
measurements. The colorimetric enzymatic method was used to measure TC,
HDL-c, and TAG. The cutoff points were those proposed to Brazilian children
and adolescents21 (link). LDL-c
was calculated using the Friedewald et al.22 (link) formula. The ultra-sensitive CRP was
measured by the turbidimetric assay and adiponectin, by the ELISA method
(Adiponectin DuoSet, R&D Systems, Inc., Minneapolis, MN, USA).
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2

Quantification of Immunological Markers in Lung and Sera

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TNF-α, and total and specific IgE levels from lung homogenates or blood sera were measured by means of enzyme-linked immunosorbent assay (ELISA) with commercially available materials. Briefly, TNF-α and total IgE levels were assayed with Mouse TNF-α DuoSet (R&D Systems) and BD OptEIA Mouse IgE ELISA Set (BD Pharmingen, San Diego, CA), respectively. Specific IgE levels were measured by modified-sandwich ELISA [17 (link)]. Briefly, the plates were coated with a purified rat anti-mouse IgE, and sera were used as test samples. Biotinylated-OVA and peroxidase-conjugated streptavidin were reacted in order. The reactions were read at 450 nm.Leptin and adiponectin levels from lung homogenates or blood sera were measured by means of ELISA with commercially available materials, Mouse Leptin and adiponectin DuoSet (R&D Systems), respectively.
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