Total protein was extracted from frozen heart tissues using RIPA buffer containing proteinase and phosphatase inhibitors (Roche; Germany). Protein lysates were separated by SDS–PAGE and transferred to PVDF membranes (Millipore; USA). The membranes were incubated with the following primary antibodies: GSK-3b, pS9-GSK-3b, NOX2 and GAPDH (Abcam; USA) overnight at 4 °C and then incubated with goat anti-rabbit IgG (Abcam; USA). The Western blot bands were detected using an ECL kit (Keygene; China). ImageJ (NIH; USA) was used to quantify the specific protein expression levels.
Gsk 3b
Manufactured by Abcam
Sourced in United States
GSK-3b is a serine/threonine protein kinase that plays a key role in the regulation of various cellular processes. It is involved in the control of glycogen synthesis, gene transcription, and cell division.
Automatically generated - may contain errors
Lab products found in correlation
Proteinase and phosphatase inhibitor, by Roche (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Ecl kit, by Keygene (1 mentions)
Gapdh, by Abcam (1 mentions)
Goat anti rabbit igg, by Abcam (1 mentions)
P egfr, by Abcam (1 mentions)
Irhom2, by Abcam (1 mentions)
Gadph, by Abcam (1 mentions)
Tgfbr2, by Abcam (1 mentions)
Horseradish peroxidase hrp labeled secondary antibody, by Santa Cruz Biotechnology (1 mentions)
β catenin, by Abcam (1 mentions)
2 protocols using gsk 3b
1
Protein Expression Analysis in Cardiac Tissue
2
Antibody Characterization for Cell Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The following antibodies were obtained from Abcam: iRhom1 (ID product code ab81342), iRhom2 (ID product code ab116139), Ki67 (ID product code ab92742), β-catenin (ID product code ab32572), Fas (ID product code ab82419), GSK3B (ID product code ab32391), Myc (ID product code ab32), TGFBR2 (ID product code ab78419), EGFR (ID product code ab52894), p-EGFR (ID product code ab40815), GADPH (ID product code ab8245), and actin (ID product code ab179467). Horseradish peroxidase (HRP)-labeled secondary antibodies (ID product code sc-2354 and sc-2357) were purchased from Santa Cruz Biotechnology, Inc.
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