Ltc4 d5

Lidocaine (2%) and proxymetacaine (0.5%) eye drops were from Moscow Endocrine Plant (Moscow, Russia) and Alcon-Couvreur (Puurs, Belgium), respectively. The Schirmer test tear strips were from Haag-Streit (Bern, Switzerland). Butylated hydroxytoluene (BHT) was from Sigma-Aldrich (St. Louis, MO, USA). The deuterated oxylipins standards 6-keto PGF1α-d4, TXB2-d4, PGF2α-d4, PGE2-d4, PGD2-d4, LTC4-d5, LTB4-d4, 5(S)-HETE-d8, 12(S)-HETE-d8, 15(S)-HETE-d8, Oleoyl Ethanolamide-d4, EPA-d5, DHA-d5 and AA-d8 were from Cayman Chemical (Ann Arbor, MI, USA). Solid-phase lipid extraction cartridge Oasis^® PRIME HLB was obtained from Waters (Eschborn, Germany). Other chemicals were from Sigma-Aldrich, Amresco (Solon, OH, USA), or Serva (Heidelberg, Germany) and were at least reagent grade.

A comprehensive analysis method for eicosanoids has been described previously (17 ). The method utilizes an LC-8060 device, consisting of a quantum triple-quadrupole mass spectrometer (Shimadzu, Kyoto, Japan) and a software method package for the simultaneous analysis of 196 products with 18 deuterium internal standards (supplemental Table S2): tetranor-PGEM-d6, 6-keto-PGF1α-d4, TXB2-d4, PGF2α-d4, PGE2-d4, PGD2-d4, PGA2-d4, LTB4-d4, 14,15-DiHET-d11, 15-HETE-d8, 12-HETE-d8, 5-HETE-d8, 11,12-EET-d11, LTC4-d5, LTD4-d5, PAF-d4, OEA-d4, and AA-d8 (Cayman Chemical, Ann Arbor, MI). The eicosanoids were identified by multiple reactions monitoring (MRM) and were analyzed using LabSolutions software (Shimadzu).