Is 1 7 diaminoheptane

BAs, 2-phenylethylamine (PEA), methylamine (MET), putrescine dihydrochloride (PUT), cadaverine dihydrochloride (CAD), tryptamine (TRY), histamine dihydrochloride (HIS), serotonin hydrochloride (5-HT), tyramine hydrochloride (TYR), dopamine hydrochloride (DA), spermidine trihydrochloride (SPD), and spermine tetrahydrochloride (SPM) were obtained from Sigma-Aldrich (St. Louis, MO, USA). IS, 1,7-diaminoheptane (DMP) was supplied by Sigma-Aldrich (Steinheim, Germany). Dansyl chloride (Dns-Cl) from Sigma-Aldrich (USA) was used as a derivatization reagent. The HPLC grade acetonitrile (ACN) was purchased from Tedia (Fairfield, CT, USA). Deionized water was produced by a MILLPAK Reagent Water System (Millipore, MA, USA). All other reagents were of analytical grade.

High purity biogenic amines of analytical standards (98% or higher): tryptamine dihydrochloride, 2-phenylethylamine dihydrochloride, putrescine dihydrochloride, cadaverine hydrochloride, histamine dihydrochloride, tyramine dihydrochloride, spermidine, spermine were purchased from Sigma-Aldrich (St. Luis, MI, USA) and Acros Organics (Geel, Belgium). The internal standard (IS) 1,7-diaminoheptane was supplied by Sigma-Aldrich. HPLC grade acetone and toluene used for reagent preparation and for extraction were purchased from Merck (Darmstadt, Germany) and Avantor Performance Materials Poland S.A. (Gliwice, Poland). Dansyl chloride from Sigma-Aldrich was used as a derivatization reagent. Perchloric acid used for extraction was supplied by Avantor Performance Materials Poland S.A. Sodium carbonate and L-proline were purchased from Sigma-Aldrich. The HPLC grade acetonitrile used as eluent in liquid chromatography was obtained from Merck. Purified through a Mili-Q Plus system water (Merck Millipore; Billerica, MA, USA) was used for mobile phase and for the standards preparation.