Cells were seeded into ibidi culture-inserts (ibidi GmbH, Martinsried, Germany) at a concentration of 10,000 cells per well. After allowing cells to attach overnight, the culture-insert was gently removed using sterile forceps. Cells were incubated with scramble, miR-29a mimic, miR-29a anti-sense inhibitor or HDAC4 RNAi. Images were taken at 0, 6, and 21 h, and superimposed using PhotoImpact (Adobe). The number of cells that migrated into the wound space were manually counted in three fields per well under a light microscope at 50× magnification. Areas were quantified by image analysis using Image J analysis.
Photoimpact
Manufactured by Adobe
Sourced in United States
PhotoImpact is a graphic design and image editing software developed by Ulead Systems, a subsidiary of Corel Corporation. It provides a range of tools and features for tasks such as photo editing, digital painting, image compositing, and web graphics creation.
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Lab products found in correlation
3 protocols using photoimpact
1
miR-29a Regulation of Cell Migration
2
Cell Proliferation Assay with Crystal Violet
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After transfection for 24 h, cells were plated into 6-well plates (1000 cells/well) and cultured in normal cultured media at 37 °C and 5% CO2 for 2 weeks. Then cells were fixed with 4% paraformaldehyde for 10 min and stained with 0.5% crystal violet for 30 min. The numbers of cells were quantified by image analysis using PhotoImpact (Adobe, San Jose, CA, USA). Each group was represented in the form of triplicates.
3
Cell Proliferation and Migration Assays
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Cell proliferation and migration assays were performed as described previously [30] . For proliferation assay, a cell concentration of 5 × 10 3 cells/well was adopted for 48 h incubation. After the incubation period, 10 μl premixed WST-1 reagents (Roche Applied Science, Mannheim, Germany) was added into each well, and the cells were incubated for 4 h in a humidified atmosphere (e.g., 37℃ 5% CO 2 ). Afterwards, the absorbance of the samples was measured at 450 nm against a background control with a reference wavelength higher than 600 nm on a microtiter plate (ELISA) reader.
For migration assay, images were taken at 0, 6, 12, and 15 h, and superimposed using PhotoImpact (Adobe). The number of cells that migrated into the wounded space were manually counted in three fields per well under a light microscope at × 40 magnifications. Areas were quantified by using Image J analysis software.
For migration assay, images were taken at 0, 6, 12, and 15 h, and superimposed using PhotoImpact (Adobe). The number of cells that migrated into the wounded space were manually counted in three fields per well under a light microscope at × 40 magnifications. Areas were quantified by using Image J analysis software.
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