Samples were fixed in 2.5% glutaraldehyde, pH 7.4, at 37°C after dissection then stored at 4°C overnight. After rinsing with PBS, the samples were post-fixed with 1% OsO4 for 1 h then rinsed with distilled water. After dehydration through an ethanol gradient, the samples were dried with a critical point dryer. They were then mounted on a stub with carbon tape and coated with gold-sputter for imaging with a Zeiss Sigma 300 FEG-SEM operating at 2 kV.
Sigma 300 feg sem
Manufactured by Zeiss
The Sigma 300 FEG-SEM is a field emission gun scanning electron microscope (FEG-SEM) manufactured by Zeiss. It is designed to provide high-resolution imaging of samples at the nanoscale level. The Sigma 300 FEG-SEM utilizes a field emission gun electron source to generate a focused electron beam, which is then scanned across the surface of the sample to generate detailed images.
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Lab products found in correlation
2 protocols using sigma 300 feg sem
1
Preparation of Biological Samples for SEM
2
SEM analysis of T. heimii cells
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Samples were taken from the T. heimii incubation bottles and 2 ml of media was filtered under low vacuum onto polycarbonate filters (0.22 µm pore size) and rinsed five times with 10 mmol.l -1 CaCl2 and then five times with 100% ethanol. Filter papers were dried in a laminar flow hood. Samples were gold coated and SEM analysis was performed at the Dunn School of Bioimaging in the Department of Physiology, University of Oxford with a Zeiss Sigma 300 FEG-SEM.
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