The following antibodies were used in the experiments: TruStain FcX anti-mouse CD16/32 (101320, BioLegend), FITC anti-mouse CD8 (A502-3B-E, ProImmune), Phycoerythrin (PE) anti-mouse CD3e (550353, BD Pharmingen), Peridinin-Chlorophyll-Protein (PerCP) anti-mouse CD19 (115531, BioLegend) and PE-Cyanine 7 anti-mouse CD4 (25-0041-82 eBioscience). SIINFEKL epitope-specific T cells were studied using APC-labeled H-2Kb/SIINFEKL pentamer (F093-84B-E, ProImmune). SVYDFFVWL (Trp2) epitope-specific T cells were studied using PE-labeled H-2Kb/SVYDFFVWL pentamer (F185-82B-E, Proimmune). SPSYVYHQF (AH1) epitope-specific T cells were studied using PE-labeled H-2Ld/SPSYVYHQF pentamer (F398-82A-E, Proimmune). Flow cytometric analysis were performed using a BD Accuri 6C Plus (BD Biosciences) or a BD LSRFortessa (BD Biosciences) flow cytometer and FlowJo software V.10 (BD Biosciences) was used for data analysis (see online supplemental figure 7 for gating strategies used in the experiments).
Peridinin chlorophyll protein percp anti mouse cd19
Manufactured by BioLegend
Peridinin-Chlorophyll-Protein (PerCP) anti-mouse CD19 is a fluorescent antibody conjugate used for the identification and enumeration of mouse CD19-positive cells in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Trustain fcx anti mouse cd16 32, by BioLegend (2 mentions)
Lsrfortessa, by BD (2 mentions)
Phycoerythrin pe anti mouse cd3e, by BD (2 mentions)
Pe cyanine 7 anti mouse cd4, by Thermo Fisher Scientific (2 mentions)
F093 84b e, by ProImmune (2 mentions)
Accuri 6c plus, by BD (2 mentions)
Fitc anti mouse cd8 a502 3b e, by ProImmune (2 mentions)
2 protocols using peridinin chlorophyll protein percp anti mouse cd19
1
Comprehensive Immune Cell Profiling
2
Multiparameter Flow Cytometry Analysis
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The following antibodies were used in the experiments: TruStain FcX™ anti-mouse CD16/32 (101320, BioLegend), FITC anti-mouse CD8 (A502-3B-E, ProImmune), Phycoerythrin (PE) antimouse CD3e (550353, BD Pharmingen), Peridinin-Chlorophyll-Protein (PerCP) anti-mouse CD19 (115531, BioLegend) and PE-Cyanine 7 anti-mouse CD4 (25-0041-82 eBioscience). SIINFEKL epitope-specific T cells were studied using APC-labelled H-2Kb/SIINFEKL pentamer (F093-84B-E, ProImmune). SVYDFFVWL (Trp2) epitope-specific T cells were studied using PE-labelled H-2Kb/SVYDFFVWL pentamer (F185-82B-E, Proimmune). SPSYVYHQF (AH1) epitope-specific T cells were studied using PE-labelled H-2Ld/SPSYVYHQF pentamer (F398-82A-E, Proimmune).
Flow cytometric analysis were performed using a BD Accuri 6C Plus (BD Biosciences) or a BD LSRFortessa™ (BD Biosciences) flow cytometer and FlowJo software v10 (BD Biosciences) was used for data analysis.
Flow cytometric analysis were performed using a BD Accuri 6C Plus (BD Biosciences) or a BD LSRFortessa™ (BD Biosciences) flow cytometer and FlowJo software v10 (BD Biosciences) was used for data analysis.
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