PEGDA 700 (Aldrich), PETMP (>95%, Aldrich), Diphenyl-(2,4,6-trimethylbenzoyl)phosphine oxide (TPO, 97%, Aldrich), 2-(2-hydroxyphenyl)-benzotriazole derivative (Tinuvin® CarboProtect®, BASF Company), 2,2′-Azobis(2-methylpropionitrile) (AIBN, 98%, Aldrich), Dulbecco’s Phosphate Buffered Saline (DPBS, Corning) without calcium and magnesium. The AIBN was mixed priorto performing the experiment to avoid degradation.
Dulbecco s phosphate
Manufactured by Corning
Dulbecco's Phosphate is a buffered saline solution commonly used in cell culture applications. It is designed to maintain the pH and osmotic balance of the cell culture environment.
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Lab products found in correlation
2 protocols using dulbecco s phosphate
1
Photocrosslinking of PEGDA Hydrogels
2
Fluorescent Imaging of Organoid Viability
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Post treatment plates were incubated at 37°C, 5% CO2 for 72 h and the wells were visualized for duct/cluster-like morphology. Media was removed and organoids in Matrigel were washed once with 100 µL/well of Dulbecco’s phosphate buffered saline (DPBS, Corning), followed by staining with Calcein AM (Sigma) in DPBS at final concentration of 4 µM for 1.5 h at 37°C in the dark. After staining, organoids were washed again with 100 µL/well of DPBS and left in fresh DPBS for live imaging. Organoids were imaged on the Operetta High-Throughput Screen imaging system using ×20 objective, brightfield and green fluorescence filters at the same 15 fields/well that were imaged before the treatment.
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