Mc170 hd digital

Overnight cultures in YPD at 37°C were transferred to glass tubes. Every 10 min 50 μl of sample was carefully taken 2 cm below the surface of the cell suspension for OD600 measurements. After 1 h the cells were washed with PBS and the sedimentation experiment was repeated. The sedimentation test with PEU382 was also performed after adjusting the pH of the cell culture to 6.5 with PBS or NaOH.
Cell aggregation of overnight cultures in YPD was observed with a Leica DM1000 microscope mounted with a MC170 HD digital camera.

For the stereological study of chondrocytes, the articular cartilage of the medial and lateral femoral condyles (FC), menisci (M), and tibial plateau (TP) was analyzed. Five fields per structure were observed in each histological section [51 (link)]. The slides were visualized under a Leica^® DM2000 LED stereological microscope and photographed with a Leica^® MC170 HD digital camera. The 36-point test system designed by STEPanizer^® was utilized, and the following parameters were determined: areal density (N_A), volume density (V_V), and surface area density (S_V) of the chondrocytes. The N_A was quantified using the equation N_A = Q/A_T, where Q represents the number of observations in a given area considering the forbidden lines, and A_T is the total area of the test system. V_V was estimated using the formula: V_V = P_P/P_T (100%), where P_P represents the number of points touching the chondrocytes and P_T is the total number of points in the system. S_V was determined according to the following equation S_V = (2 × I)/L_T, where I represents the number of intersections touching the structure, and L_T is the total length of the lines of the 36-point test system.