Custom carbon fiber electrodes were made in-house and inserted into a glass pipette (50–100 μm in length, 7 μm diameter, T-795, GoodFellow Corp. Coraopolis, PA). Electrodes were cleaned with bleach to improve sensitivity and pre-calibrated with dopamine. Electrodes were inserted 50–100 μm below the surface of acute striatal slices. To distinguish dopamine from other catecholamines (Rice et al., 1997 (link)), extracellular dopamine levels were monitored as current generated by application of a triangular waveform (−0.4 to +1.0 to −0.4 V versus Ag/AgCl at 200 V/sec scan speed) every 100 msec (10 Hz). The peak of oxidation current at +400mV was measured after background current subtraction. All data were acquired using a MultiClamp 700B amplifier (Molecular Devices, Sunnyvale, CA) with 4kHz high-pass filter and digitized in Digidata1550B (Molecular Devices) at 10kHz. Electrical stimulation was provided by a 50 μm diameter twisted tungsten wire stimulating electrode (A-M Systems, Sequim, WA) placed in close proximity to the carbon fiber electrode. Electrical stimulation was also applied at 20 Hz for 1sec (10V, 200 μs pulse duration) delivered from an isolated pulse stimulator (Model 2100, A-M systems).
Twisted tungsten wire stimulating electrode
Manufactured by A-M Systems
The twisted tungsten wire stimulating electrode is a lab equipment product designed for electrical stimulation applications. It features a twisted design of tungsten wire that provides a durable and flexible electrode for controlled stimulation in various research and experimental settings.
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2 protocols using twisted tungsten wire stimulating electrode
1
Electrochemical Monitoring of Striatal Dopamine
2
Electrochemical Monitoring of Striatal Dopamine
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Custom carbon fiber electrodes were made in-house and inserted into a glass pipette (50–100 μm in length, 7 μm diameter, T-795, GoodFellow Corp. Coraopolis, PA). Electrodes were cleaned with bleach to improve sensitivity and pre-calibrated with dopamine. Electrodes were inserted 50–100 μm below the surface of acute striatal slices. To distinguish dopamine from other catecholamines (Rice et al., 1997 (link)), extracellular dopamine levels were monitored as current generated by application of a triangular waveform (−0.4 to +1.0 to −0.4 V versus Ag/AgCl at 200 V/sec scan speed) every 100 msec (10 Hz). The peak of oxidation current at +400mV was measured after background current subtraction. All data were acquired using a MultiClamp 700B amplifier (Molecular Devices, Sunnyvale, CA) with 4kHz high-pass filter and digitized in Digidata1550B (Molecular Devices) at 10kHz. Electrical stimulation was provided by a 50 μm diameter twisted tungsten wire stimulating electrode (A-M Systems, Sequim, WA) placed in close proximity to the carbon fiber electrode. Electrical stimulation was also applied at 20 Hz for 1sec (10V, 200 μs pulse duration) delivered from an isolated pulse stimulator (Model 2100, A-M systems).
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